Journal article
In vivo molecular engineering of the urethra for treatment of stress incontinence using novel biomimetic proteoglycans
Journal of biomedical materials research. Part B, Applied biomaterials, v 107(7), pp 2409-2418
Oct 2019
PMID: 30784181
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Stress urinary incontinence (SUI), a serious condition which affects ~56% of postmenopausal women, is the involuntary leakage of urine through urethra during physical activity that causes an increase in abdominal pressure. SUI is associated with a decrease in compliance and volume of urethral tissue, likely due to a reduced proteoglycan: collagen ratio in the extracellular matrix and collagen disorganization. Here, we investigated the use of biomimetic proteoglycans (BPGs) to molecularly engineer urethral tissue of New Zealand White rabbits to examine biocompatibility in vivo. BPG concentrations of 50 mg/mL (n = 6, 1 week) and 200 mg/mL (n = 6, 1 week and n = 6, 6 weeks) dissolved in 1× phosphate‐buffered saline (PBS) were injected transurethrally using a 9 French cystoscope, and were compared to PBS‐injected controls (n = 3, 1 week) and non‐injected controls (n = 2, 1 week). Urethral compression pressure measurements confirm BPG injections did not modify normal urethral pressure, as intended. Histological assessment demonstrated biological tolerance of BPGs in urethra and no inflammatory response was detected after 1 and 6 weeks compared to non‐injected controls. Confocal imaging of fluorescently‐labeled BPG injected urethral specimens demonstrated the integration of BPGs into the interstitial connective tissue and confirmed they were still present after 6 weeks. A general decrease of collagen density was exhibited near injection sites which may be due to increased hydration induced by BPGs. Injection of BPGs is a novel approach that demonstrates potential as molecular treatment for SUI and may be able to reverse some of the degenerative tissue changes of individuals affected by this condition. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: 00B: 000–000, 2019. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2409–2418, 2019.
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Details
- Title
- In vivo molecular engineering of the urethra for treatment of stress incontinence using novel biomimetic proteoglycans
- Creators
- Alicia S. Kriete - Drexel UniversityNatasha Ginzburg - Drexel UniversityNima Shah - Drexel UniversityRichard B. Huneke - Drexel UniversityEmily Reimold - Drexel UniversityKatsiaryna Prudnikova - Drexel UniversityOwen Montgomery - Drexel UniversityJ. Steve Hou - Drexel UniversityEvan R. Phillips - Drexel UniversityMichele S. Marcolongo - Drexel University
- Publication Details
- Journal of biomedical materials research. Part B, Applied biomaterials, v 107(7), pp 2409-2418
- Publisher
- John Wiley & Sons, Inc
- Number of pages
- 10
- Grant note
- Wallace H. Coulter Foundation
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; School of Biomedical Engineering, Science, and Health Systems; Materials Science and Engineering; College of Nursing and Health Professions
- Web of Science ID
- WOS:000483702000021
- Scopus ID
- 2-s2.0-85061913586
- Other Identifier
- 991019168363304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials