Journal article
Increased Levels of Methylated Intermediates of Phosphatidylcholine Lead to Enhanced Phospholipase D Activity
Neurochemical research, v 23(8), pp 1099-1105
Aug 1998
PMID: 9704600
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Previous work from this laboratory and others has shown that neurotransmitters can activate phospholipase D. Unlike the phospholipase C that specifically hydrolyzes inositol-containing phospholipids, phospholipase D in neuronal tissue specifically hydrolyzes phosphatidylcholine. One route for the synthesis of phosphatidylcholine, is via methylation of phosphatidylethanolamine. Using an in vitro assay, we have previously shown that methylated intermediates are also good substrates for phospholipase D (1). In this manuscript we demonstrate that these intermediates are also substrates in the intact PC 12 cells. Cells incubated with methyl and dimethylethanolamine incorporate more [3H]palmitic acid into the corresponding phospholipid, phosphatidyl-N-methylethanolamine and phosphatidyl-N,N-dimethylethanolamine. In these cells bradykinin causes a greater increase in [3H]phosphatidylethanol production. Elevated levels of [3H]phosphatidylcholine do not enhance bradykinin-stimulated [3H]phosphatidylethanol production, therefore, this effect is specific for the methylated intermediates. Finally, this effect is not due to some generalized enhancement of receptor coupling because incubation of the cells with methylethanolamine does not lead to an increase in bradykinin stimulated inositol phosphate production.
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Details
- Title
- Increased Levels of Methylated Intermediates of Phosphatidylcholine Lead to Enhanced Phospholipase D Activity
- Creators
- Thomas Jacobs - Department of Pharmacology, Allegheny University of Health Sciences MCP ♦ Hahnemann School of Medicine 3200 Henry Avenue Philadelphia PA 19129Brent Passarello - Department of Pharmacology, Allegheny University of Health Sciences MCP ♦ Hahnemann School of Medicine 3200 Henry Avenue Philadelphia PA 19129Joel Horwitz - Department of Pharmacology, Allegheny University of Health Sciences MCP ♦ Hahnemann School of Medicine 3200 Henry Avenue Philadelphia PA 19129
- Publication Details
- Neurochemical research, v 23(8), pp 1099-1105
- Publisher
- Kluwer Academic Publishers-Plenum Publishers; New York
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000075089000011
- Scopus ID
- 2-s2.0-0031828234
- Other Identifier
- 991014878624304721
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InCites Highlights
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Neurosciences