Journal article
Increased Vulnerability of NFH-LacZ Transgenic Mouse to Traumatic Brain Injury-Induced Behavioral Deficits and Cortical Damage
Journal of cerebral blood flow and metabolism, v 19(7), pp 762-770
Jul 1999
PMID: 10413031
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The authors evaluated the neurobehavioral and neuropathologic sequelae after traumatic brain injury (TBI) in transgenic (TG) mice expressing truncated high molecular weight neurofilament (NF) protein fused to beta-galactosidase (NFH-LacZ), which develop Lewy body-like NF-rich inclusions throughout the CNS. TG mice and their wild-type (WT) littermates were subjected to controlled cortical impact brain injury (TG, n=19; WT, n=17) or served as uninjured controls (TG, n =11; WT, n =11). During a 3-week period, mice were evaluated with an array of neuromotor function tests including neuroscore, beam balance, and both fast and slow acceleration rotarod. Brain-injured WT and TG mice showed significant motor dysfunction until 15 days and 21 days post-injury, respectively (P < .025). Compared with brain-injured WT mice, brain-injured TG mice had significantly greater motor dysfunction as assessed by neuroscore (P < .01) up to and including 15 days post-injury. Similarly, brain-injured TG mice performed significantly worse than brain-injured WT mice on slow acceleration rotarod at 2, 8, and 15 days post-injury (P < .05), and beam balance over 2 weeks post-injury (P < .01). Histopathologic analysis showed significantly greater tissue loss in the injured hemisphere in TG mice at 4 weeks post-injury (P < .01). Together these data show that NFH-LacZ TG mice are more behaviorally and histologically vulnerable to TBI than WT mice, suggesting that the presence of NF-rich inclusions may exacerbate neuromotor dysfunction and cell death after TBI.
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Details
- Title
- Increased Vulnerability of NFH-LacZ Transgenic Mouse to Traumatic Brain Injury-Induced Behavioral Deficits and Cortical Damage
- Creators
- Michio Nakamura - University of PennsylvaniaKathryn E. Saatman - Department of Neurosurgery, Philadelphia, Pennsylvania, U.S.AJames E. Galvin - Allegheny University of the Health SciencesUwe Scherbel - Department of Neurosurgery, Philadelphia, Pennsylvania, U.S.ARamesh Raghupathi - Department of Neurosurgery, Philadelphia, Pennsylvania, U.S.AJohn Q. Trojanowski - University of PennsylvaniaTracy K. McIntosh - University of Pennsylvania
- Publication Details
- Journal of cerebral blood flow and metabolism, v 19(7), pp 762-770
- Publisher
- SAGE Publications
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000084948800006
- Scopus ID
- 2-s2.0-0033509025
- Other Identifier
- 991019168078804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Endocrinology & Metabolism
- Hematology
- Neurosciences