Journal article
Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor
Applied biochemistry and biotechnology, v 175(8), pp 3673-3682
01 Apr 2015
PMID: 25820358
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The antibiotic production and spore formation process in Streptomyces coelicolor need complex decision making processes by several regulatory units. These regulatory units are involved in both primary and secondary metabolism. As a result, most regulators have several functions, and those are worthwhile themes to study about different functions of a known regulator. In this study, a deletion mutant of ndgR, which encodes the nitrogen-dependent growth regulator, was examined by the cell viability test, TEM, and growth in N-acetylglucosamine/asparagine (GlcNAc/Asn) liquid medium. The results of the study show that NdgR is also involved in the structure of the cell membrane affecting survival under physical shocks. Deletion of ndgR leads to abnormal cell membrane resulting in the vulnerable cells to physical stress caused by shaking with beads in liquid culture condition. This empirical observation is the first meaningful explanation to why ndgR mutant could not grow well in a liquid minimal medium due to the defect of N-acetylglucosamine (GlcNAc) utilization and phospholipid synthesis.
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Details
- Title
- Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor
- Creators
- Bo-Rahm Lee - Seoul National UniversityDa-Hye Yi - Konkuk UniversityEunjung SongShashi Kant Bhatia - Konkuk UniversityJu Hee Lee - Konkuk UniversityYun-Gon Kim - Soongsil UniversitySung-Hee Park - Seoul National UniversityYoo Kyung Lee - Korea Polar Research InstituteByung-Gee Kim - Seoul Natl Univ, Sch Chem & Biol Engn, Seoul 151742, South KoreaYung-Hun Yang - Konkuk University
- Publication Details
- Applied biochemistry and biotechnology, v 175(8), pp 3673-3682
- Publisher
- Humana Press Inc
- Number of pages
- 10
- Grant note
- PE14030 / KOPRI; Korea Polar Research Institute of Marine Research Placement (KOPRI) 10049674 / MOTIE/KEIT; Ministry of Trade, Industry & Energy (MOTIE), Republic of Korea NRF-2013R1A1A2A10004690 / Basic Science Research Program through National Research Foundation of Korea (NRF) - Ministry of Education; Ministry of Education (MOE), Republic of Korea; National Research Foundation of Korea 20133030000300 / Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) - Korea Government Ministry of Trade, Industry and Energy
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000352081500008
- Scopus ID
- 2-s2.0-84940009235
- Other Identifier
- 991021229887704721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biotechnology & Applied Microbiology