Journal article
Increased mitochondrial mass in apoptotic HIV-specific CD8+ T cells
Journal of neurovirology, Vol.12
01 May 2006
Abstract
What governs the increased apoptosis sensitivity of HIV-specific CD8+ T cells is poorly understood. Here we report the possible involvement of mitochondria and filamentous actin in this process. We report here that significantly higher mitochondrial mass (MM) is found in HIV- (77 plus or minus 7.2%, n = 15) compared to CMV-specific CD8+ T cells (50 plus or minus 5.9%, n = 13, p < 0.0002). Furthermore, CD95/Fas-induced apoptosis was remarkably increased in MMHigh (41.4 plus or minus 4% annexin V+, n = 9) compared to MMLow (8.2 plus or minus 1.7% annexin V+, n = 9, p < 0.0001) CD8+ T cells from HIV+ patients. Proapoptotic HIV-specific CD8+ T cells exhibited a CD38+ Bcl-2Low MMHigh phenotype. Mitochondria colocalized with CD95/Fas capping, very early during CD95/Fas-induced apoptosis of HIV-specific CD8+ T cells, suggesting their active role as an amplification step for this apoptosis and a potential involvement of the cytoskeleton in this process. Indeed, disruption of actin by cytochalasin D (cytD) dramatically suppressed CD95/Fas-induced apoptosis of HIV-specific CD8+ T cells (40.8 plus or minus 5% versus 21.3 plus or minus 4.6% annexin V, n = 13, p < 0.02, in the absence or presence of cytD respectively). This effect was independent of memory and activation status of these cells. The data above reveal mitochondria and filamentous actin as potential mediators of HIV-specific CD8+ T cell apoptosis further clarifying the molecular mechanisms governing this process.
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Details
- Title
- Increased mitochondrial mass in apoptotic HIV-specific CD8+ T cells
- Creators
- C PetrovasY MuellerS R AltorkP SklarK MounzerJ AltmanP Katsikis
- Publication Details
- Journal of neurovirology, Vol.12
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Infectious Diseases (and HIV Medicine)
- Identifiers
- 991019170467804721