Journal article
Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations
Nanomedicine, v 11(1), pp 19-29
01 Jan 2015
PMID: 25038496
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Safe and efficient delivery of therapeutic cells to sites of injury/disease in the central nervous system is a key goal for the translation of clinical cell transplantation therapies. Recently, 'magnetic cell localization strategies' have emerged as a promising and safe approach for targeted delivery of magnetic particle (MP) labeled stem cells to pathology sites. For neuroregenerative applications, this approach is limited by the lack of available neurocompatible MPs, and low cell labeling achieved in neural stem/precursor populations. We demonstrate that high magnetite content, self-sedimenting polymeric MPs [unfunctionalized poly(lactic acid) coated, without a transfecting component] achieve efficient labeling (>= 90%) of primary neural stem cells (NSCs)-a 'hard-to-label' transplant population of major clinical relevance. Our protocols showed high safety with respect to key stem cell regenerative parameters. Critically, labeled cells were effectively localized in an in vitro flow system by magnetic force highlighting the translational potential of the methods used.
From the Clinical Editor: Utilizing self-sedimenting polymeric magnetic particles, the authors demonstrate an efficient method for magnetically labeling primary neural stem cells for magnetic localization in the central nervous system. (C) 2015 Elsevier Inc. All rights reserved.
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Details
- Title
- Increasing magnetite contents of polymeric magnetic particles dramatically improves labeling of neural stem cell transplant populations
- Creators
- Christopher F. Adams - Keele UniversityAhmad Rai - Drexel UniversityGregor Sneddon - Heriot-Watt UniversityHumphrey H. P. Yiu - Heriot Watt Univ, Dept Chem Engn, Sch Engn & Phys Sci, Edinburgh, Midlothian, ScotlandBoris Polyak - Drexel UniversityDivya M. Chari - Keele University
- Publication Details
- Nanomedicine, v 11(1), pp 19-29
- Publisher
- Elsevier
- Number of pages
- 11
- Grant note
- Engineering and Physical Sciences Research Council Doctoral Training Centre in Regenerative Medicine; UK Research & Innovation (UKRI); Engineering & Physical Sciences Research Council (EPSRC) R01HL107771 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) BB/J017590/1 / Biotechnology and Biological Sciences Research Council; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) BB/J017590/1 / BBSRC; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) Biotechnology and Biological Sciences Research Council, UK; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC) 1119377 / Engineering and Physical Sciences Research Council; UK Research & Innovation (UKRI); Engineering & Physical Sciences Research Council (EPSRC) 5R01HL107771 / National Heart, Lung and Blood Institute; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:000346846500002
- Scopus ID
- 2-s2.0-84919844469
- Other Identifier
- 991019169787804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Medicine, Research & Experimental
- Nanoscience & Nanotechnology