Journal article
Inducing Immunomodulatory Effects on Human Macrophages by Multifunctional NCO-sP(EO-stat-PO)/Gelatin Hydrogel Nanofibers
ACS biomaterials science & engineering, v 7(7), pp 3166-3178
12 Jul 2021
PMID: 34114792
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Endowing materials and scaffolds with immunomodulatory properties has evolved into a very active field of research. However, combining such effects with multifunctionality regarding cell adhesion and manipulation is still challenging due to the intricate nature of cell-substrate interactions that require fine-tuning of scaffold properties. Here, we reported electrospinning of a well-known biopolymer, gelatin, together with six-arm star-shaped poly(ethylene oxidestat-propylene oxide) prepolymer with isocyanate end groups (NCO-sP(EO-stat-PO)) as a reactive prepolymer cross-linker. Covalent coupling of two components during and after processing yielded a network of hydrogel fibers that was remarkably stable under aqueous and also proteolytic conditions without the need for extra cross-linking, with a significant increase in stability with increasing NCOsP(EO-stat-PO) content. When seeded with human macrophages, cells adhered and spread on the fibers and were found highly viable after 7 days of culture across all scaffolds. Furthermore, hybrid fibrous meshes upregulated the expression of a prohealing gene, CD206, while downregulating proinflammatory genes, IL-1 beta and IL-8. Markedly, NCO-sP(EO-stat-PO)-rich samples induced a significantly reduced release of proinflammatory cytokines, IL-1 beta, IL-6, and IL-8. Finally, we successfully conjugated IL-4 to NCO-sP(EO-stat-PO) that effectively steered macrophages into a prohealing M2 type, demonstrating additional and robust control over the immunomodulatory feature of the scaffolds.
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Details
- Title
- Inducing Immunomodulatory Effects on Human Macrophages by Multifunctional NCO-sP(EO-stat-PO)/Gelatin Hydrogel Nanofibers
- Creators
- Mehmet Berat Taskin - University of WürzburgTina Tylek - University of WürzburgCarina Blum - University of WürzburgChristoph Bohm - Univ Wurzburg, Dept Funct Mat Med & Dent, Inst Biofabricat & Funct Mat, D-97070 Wurzburg, GermanyChristina Wiesbeck - University of WürzburgJuergen Groll - Univ Wurzburg, Dept Funct Mat Med & Dent, Inst Biofabricat & Funct Mat, D-97070 Wurzburg, Germany
- Publication Details
- ACS biomaterials science & engineering, v 7(7), pp 3166-3178
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 13
- Grant note
- European Fund for Regional Development (EFRE Bayern)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000674162000026
- Scopus ID
- 2-s2.0-85109003128
- Other Identifier
- 991019292228404721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Materials Science, Biomaterials