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Journal article
Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts
Molecular and cellular biology, v 11(10), pp 4943-4951
01 Oct 1991
PMID: 1922027
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Abstract
A DNA-binding factor with properties of NF-kappa B and another similar activity are rapidly induced when growth-arrested BALB/c 3T3 cells are stimulated with serum growth factors. Induction of these DNA-binding activities is not inhibited by pretreatment of quiescent cells with the protein synthesis inhibitor cycloheximide. Interestingly, the major NF-kappa B-like activity is not detected in nuclear extracts of proliferating cells, and thus its expression appears to be limited to the G0-to-G1 transition in 3T3 cells. These DNA-binding activities bind many of the expected NF-kappa B target sequences, including elements in the class I major histocompatibility complex and human immunodeficiency virus enhancers, as well as a recently identified NF-kappa B binding site upstream of the c-myc gene. Furthermore, both the class I major histocompatibility complex and c-myc NF-kappa B binding sites confer inducibility on a minimal promoter in 3T3 cells stimulated with serum growth factors. The results demonstrate that NF-kappa B-like activities are immediate-early response proteins in 3T3 cells and suggest a role for these factors in the G0-to-G1 transition.
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Details
- Title
- Induction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts
- Creators
- A S Baldwin, Jr - Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill 27599-7295J C Azizkhan - University of North Carolina at Chapel HillD E Jensen - University of North Carolina at Chapel HillA A Beg - University of North Carolina at Chapel HillL R Coodly - University of North Carolina at Chapel Hill
- Publication Details
- Molecular and cellular biology, v 11(10), pp 4943-4951
- Publisher
- American Society for Microbiology (ASM)
- Grant note
- CA09156 / NCI NIH HHS 1 RO1 CA52515-01 / NCI NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1991GJ40100016
- Scopus ID
- 2-s2.0-0025941525
- Other Identifier
- 991020200754404721
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- Web of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology