Files and links (1)
Accepted (AM)Open Access (License Unspecified), Open
Journal article
Induction of protective immunity by vaccination against Chlamydia trachomatis using the major outer membrane protein adjuvanted with CpG oligodeoxynucleotide coupled to the nontoxic B subunit of cholera toxin
Vaccine, v 27(44), pp 6239-6246
19 Oct 2009
PMID: 19686693
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The present study was undertaken to test the efficacy of immunization with the native major outer membrane protein (nMOMP) of
Chlamydia trachomatis mouse pneumonitis (MoPn) serovar in combination with a novel immunostimulatory adjuvant consisting of CpG oligodeoxynucleotide (ODN) linked to the nontoxic B subunit of cholera toxin (CTB-CpG) to elicit a protective immune response to
C. trachomatis. High levels of
Chlamydia-specific IgG antibodies were detected in the sera from BALB/c mice immunized intramuscularly and subcutaneously (i.m.
+
s.c.) with the nMOMP/CTB-CpG vaccine or with nMOMP adjuvanted with a mixture of CT and CpG ODN (CT
+
CpG). Further, these immunization schemes gave rise to significant T-cell-mediated
Chlamydia-specific immune responses. No
Chlamydia-specific humoral or cell-mediated immune responses were detected in the control mice vaccinated with ovalbumin together with either CTB-CpG or CT
+
CpG. Following an intranasal challenge with
C. trachomatis the groups of mice immunized with nMOMP plus CTB-CpG, CT
+
CpG or live
C. trachomatis were found to be protected based on their change in body weight and lung weight as well as number of inclusion forming unit recovered from the lungs, as compared with control groups immunized with ovalbumin plus either adjuvants. Interestingly, IFN-γ-producing CD4
+, but not CD8
+, T-cells showed a significant correlation with the outcomes of the challenge. In conclusion, nMOMP in combination with the novel adjuvant CTB-CpG elicited a significant antigen-specific antibody and cell-mediated immune responses as well as protection against a pulmonary challenge with
C. trachomatis.
Metrics
Details
- Title
- Induction of protective immunity by vaccination against Chlamydia trachomatis using the major outer membrane protein adjuvanted with CpG oligodeoxynucleotide coupled to the nontoxic B subunit of cholera toxin
- Creators
- Chunmei Cheng - University of California, IrvineIlham Bettahi - University of California, IrvineMaria I. Cruz-Fisher - University of California, IrvineSukumar Pal - University of California, IrvinePooja Jain - University of California, IrvineZhenyu Jia - University of California, IrvineJan Holmgren - University of GothenburgAli M. Harandi - University of GothenburgLuis M. de la Maza - University of California, IrvineSahlgrenska akademin
- Publication Details
- Vaccine, v 27(44), pp 6239-6246
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000271486100021
- Scopus ID
- 2-s2.0-70349973772
- Other Identifier
- 991020545227704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology
- Medicine, Research & Experimental