Journal article
Influence of growth phase on adhesion kinetics of Escherichia coli D21g
Applied and environmental microbiology, v 71(6), pp 3093-3099
01 Jun 2005
PMID: 15933006
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The influence of bacterial growth stage and the evolution of surface macromolecules on cell adhesion have been examined by using a mutant of Escherichia coli K-12. To better understand the adhesion kinetics of bacteria in the mid-exponential and stationary growth phases under flow conditions, deposition experiments were conducted in a well-controlled radial stagnation point flow (RSPF) system. Complementary cell characterization techniques were conducted in combination with the RSPF experiments to evaluate the hydrophobicity, electrophoretic mobility, size, and titratable surface charge of the cells in the two growth phases considered. It was observed that cells in stationary phase were notably more adhesive than those in mid-exponential phase. This behavior is attributed to the high degree of local charge heterogeneity on the outer membranes of stationary-phase cells, which results in decreased electrostatic repulsion between the cells and a quartz surface. The mid-exponential-phase cells, on the other hand, have a more uniform charge distribution on the outer membrane, resulting in greater electrostatic repulsion and, subsequently, less adhesion. Our results suggest that the macromolecules responsible for this phenomenon are outer membrane-bound proteins and lipopolysaccharide-associated functional groups.
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Details
- Title
- Influence of growth phase on adhesion kinetics of Escherichia coli D21g
- Creators
- Sharon L Walker - University of California, RiversideJane E Hill - Yale UniversityJeremy A Redman - California State University, Long BeachMenachem Elimelech - Yale University
- Publication Details
- Applied and environmental microbiology, v 71(6), pp 3093-3099
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- College of Engineering
- Web of Science ID
- WOS:000229790900036
- Scopus ID
- 2-s2.0-20444390064
- Other Identifier
- 991021230008604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology