Journal article
Influence of hyaluronic acid modification on CD44 binding towards the design of hydrogel biomaterials
Biomaterials, v 222, pp 119451-119451
01 Nov 2019
PMID: 31480001
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Hyaluronic acid (HA) is a linear polysaccharide of D-glucuronic acid and N-acetyl-D-glucosamine that is native to many tissues and interacts with cells via cell-surface receptors (e.g., CD44). HA has been extensively explored as a chemically-modified macromer for crosslinking into biomaterials, such as hydrogels and macroporous scaffolds. However, the influence of the extent and type of HA modification on its binding to CD44 is not well understood or quantified. To address this, we modified HA at either the carboxylic acid or the primary alcohol with various chemical groups (e.g., norbomenes, methacrylates) and magnitudes (similar to 10, 20, or 40% of disaccharides) and then characterized binding in both soluble and hydrogel forms. HA binding to CD44 immobilized on plates or presented by cells was influenced by the extent and type of its modification, where increased modification (i.e., similar to 40%) generally decreased binding. The adhesion of CD44-modified beads to hydrogels as measured by atomic force microscopy revealed a similar trend, particularly with decreased adhesion with hydrophobic modifications to the carboxylic acid. Further, the chondrogenesis of mesenchymal stromal cells when encapsulated in hydrogels fabricated from modified HA macromers was reduced at high modification, behaving similarly to inert hydrogel controls. This work suggests that the types and extents of modification of polysaccharides are important factors that should be considered in preserving their biological function when processed as hydrogels.
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Details
- Title
- Influence of hyaluronic acid modification on CD44 binding towards the design of hydrogel biomaterials
- Creators
- Mi Y. Kwon - University of PennsylvaniaChao Wang - Drexel UniversityJonathan H. Galarraga - University of PennsylvaniaEllen Pure - University of PennsylvaniaLin Han - Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USAJason A. Burdick - University of Pennsylvania
- Publication Details
- Biomaterials, v 222, pp 119451-119451
- Publisher
- Elsevier
- Number of pages
- 9
- Grant note
- National Science Foundation Graduate Research Fellowships; National Science Foundation (NSF) R01 EB008722; T32 AR007132 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01EB008722 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB) T32AR007132 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000488138700007
- Scopus ID
- 2-s2.0-85071516381
- Other Identifier
- 991019169529004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Engineering, Biomedical
- Materials Science, Biomaterials