Journal article
Ingestion and inactivation of bacteriophages by Tetrahymena
The Journal of eukaryotic microbiology, v 55(1), pp 44-50
01 Jan 2008
PMID: 18251802
Abstract
Abiotic factors are thought to be primarily responsible for the loss of bacteriophages from the environment, but ingestion of phages by heterotrophs may also play a role in their elimination. Tetrahymena thermophila has been shown to ingest and inactivate bacteriophage T4 in co-incubation experiments. In this study, other Tetrahymena species were co-incubated with T4 with similar results. In addition, T. thermophila was shown to inactivate phages T5 and lambda in co-incubations. Several approaches, including direct visualization by electron microscopy, demonstrated that ingestion is required for T4 inactivation. Mucocysts were shown to have no role in the ingestion of T4. When S-35-labeled T4 were fed to T. thermophila in a pulse-chase experiment, the degradation of two putative capsid proteins, gp23(*) and hoc, was observed. In addition, a polypeptide with the apparent molecular mass of 52 kDa was synthesized. This suggests that Tetrahymena can use phages as a minor nutrient source in the absence of bacteria.
Metrics
Details
- Title
- Ingestion and inactivation of bacteriophages by Tetrahymena
- Creators
- Wendy Hennemuth - Edinboro UniversityLaura S. Rhoads - State University of New York at PotsdamHenry Eichelberger - Binghamton UniversityMiki Watanabe - Edinboro UniversityKevin M. Van Bell - Edinboro UniversityLei Ke - Edinboro UniversityHyesuk Kim - Edinboro UniversityGiang Nguyen - Edinboro UniversityJeremy D. Jonas - Edinboro UniversityDerek Veith - Edinboro UniversityCraig T. Van Bell - Edinboro UniversityMarisa Watanabe - Graphic Design
- Publication Details
- The Journal of eukaryotic microbiology, v 55(1), pp 44-50
- Publisher
- Wiley
- Number of pages
- 7
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Graphic Design
- Web of Science ID
- WOS:000252775700007
- Scopus ID
- 2-s2.0-38649140154
- Other Identifier
- 991019173558004721
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Microbiology