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Ingestion and inactivation of bacteriophages by Tetrahymena
Journal article   Open access   Peer reviewed

Ingestion and inactivation of bacteriophages by Tetrahymena

Wendy Hennemuth, Laura S. Rhoads, Henry Eichelberger, Miki Watanabe, Kevin M. Van Bell, Lei Ke, Hyesuk Kim, Giang Nguyen, Jeremy D. Jonas, Derek Veith, …
The Journal of eukaryotic microbiology, v 55(1), pp 44-50
01 Jan 2008
DOI: https://doi.org/10.1111/j.1550-7408.2007.00303.x
PMID: 18251802
url
https://doi.org/10.1111/j.1550-7408.2007.00303.xView
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze),  Open

Abstract

Life Sciences & Biomedicine Microbiology Science & Technology
Abiotic factors are thought to be primarily responsible for the loss of bacteriophages from the environment, but ingestion of phages by heterotrophs may also play a role in their elimination. Tetrahymena thermophila has been shown to ingest and inactivate bacteriophage T4 in co-incubation experiments. In this study, other Tetrahymena species were co-incubated with T4 with similar results. In addition, T. thermophila was shown to inactivate phages T5 and lambda in co-incubations. Several approaches, including direct visualization by electron microscopy, demonstrated that ingestion is required for T4 inactivation. Mucocysts were shown to have no role in the ingestion of T4. When S-35-labeled T4 were fed to T. thermophila in a pulse-chase experiment, the degradation of two putative capsid proteins, gp23(*) and hoc, was observed. In addition, a polypeptide with the apparent molecular mass of 52 kDa was synthesized. This suggests that Tetrahymena can use phages as a minor nutrient source in the absence of bacteria.

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Domestic collaboration
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Microbiology
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