Journal article
Inhibition of NF-κB activity by HIV-1 Vpr is dependent on Vpr binding protein
Journal of cellular physiology, v 228(4), pp 781-790
Apr 2013
PMID: 23001849
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Numerous studies have reported that Vpr alters NF-κB signaling in various cell types, however, the findings have been largely conflicting with reports of both stimulatory and inhibitory effects of Vpr. Our aim was to investigate the role of Vpr signaling in myeloid cells using an adenovirus based expression and indicator system. Our results show that Vpr is inhibitory to NF-κB, however, this effect is dependant on the particular manner of NF-κB stimulation. Consistent with this notion, we report that Vpr has inhibitory effects that are specific to the TNF-α pathway, but not affecting the LPS pathway, suggesting that differential targets of Vpr may exist for NF-κB regulation. Further, we identify VprBP as one possible cellular component of Vpr’s regulation of IκBα in response to TNF-α stimulation. We did not identify such a role for HSP27, which instead seems to inhibit Vpr functions. Chronically HIV-1 infected U1 cells with knockdown constructs for Vpr were unexpectedly less responsive to TNF-α mediated viral replication, perhaps suggesting that other HIV-1 components may antagonize these anti-NF-κB effects in infected cells. We hypothesize that Vpr may serve an important role in the context of viral infection and immune function
in vivo,
through its selective inhibition of NF-κB pathways.
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Details
- Title
- Inhibition of NF-κB activity by HIV-1 Vpr is dependent on Vpr binding protein
- Creators
- Michael Kogan - Department of Neuroscience, Center for Neurovirology. Temple University School of Medicine, 3500 North Broad Street. Medical Education and Research Building, 7th Floor. Philadelphia, PA 19140Satish Deshmane - Department of Neuroscience, Center for Neurovirology. Temple University School of Medicine, 3500 North Broad Street. Medical Education and Research Building, 7th Floor. Philadelphia, PA 19140Bassel Sawaya - Department of Neurology. Temple University School of Medicine, 3401 N. Broad Street. Parkinson Pavilion, Suite C525. Philadelphia, PA 19140Ed J Gracely - Family, Community, and Preventive Medicine. Drexel University College of Medicine, 2900 Queen Lane. Philadelphia. Philadelphia PA 19129Kamel Khalili - Department of Neuroscience, Center for Neurovirology. Temple University School of Medicine, 3500 North Broad Street. Medical Education and Research Building, 7th Floor. Philadelphia, PA 19140Jay Rappaport - Department of Neuroscience, Center for Neurovirology. Temple University School of Medicine, 3500 North Broad Street. Medical Education and Research Building, 7th Floor. Philadelphia, PA 19140
- Publication Details
- Journal of cellular physiology, v 228(4), pp 781-790
- Publisher
- Wiley
- Grant note
- R01 MH090910 || MH / National Institute of Mental Health : NIMH
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- MD (Doctor of Medicine) Program
- Web of Science ID
- WOS:000312945500016
- Scopus ID
- 2-s2.0-84871699358
- Other Identifier
- 991014878080804721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Cell Biology
- Physiology