Journal article
Inhibition of Oncogenic and Activated Wild-Type ras-p21 Protein-Induced Oocyte Maturation by Peptides from the Guanine-Nucleotide Exchange Protein, SOS, Identified from Molecular Dynamics Calculations. Selective Inhibition of Oncogenic ras-p21
Journal of protein chemistry, v 18(8), pp 875-879
01 Nov 1999
PMID: 10839624
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In the preceding paper we performed molecular dynamics calculations of the average structures of the SOS protein bound to wild-type and oncogenic ras-p21. Based on these calculations, we have identified four major domains of the SOS protein, consisting of residues 631-641, 676-691, 718-729, and 994-1004, which differ in structure between the two complexes. We have now microinjected synthetic peptides corresponding to each of these domains into Xenopus laevis oocytes either together with oncogenic (Val 12)-p21 or into oocytes subsequently incubated with insulin. We find that the first three peptides inhibit both oncogenic and wild-type p21-induced oocyte maturation, while the last peptide much more strongly inhibits oncogenic p21 protein-induced oocyte maturation. These results suggest that each identified SOS region is involved in ras-stimulated signal transduction and that the 994-1004 domain is involved uniquely with oncogenic ras-p21 signaling.[PUBLICATION ABSTRACT]
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Details
- Title
- Inhibition of Oncogenic and Activated Wild-Type ras-p21 Protein-Induced Oocyte Maturation by Peptides from the Guanine-Nucleotide Exchange Protein, SOS, Identified from Molecular Dynamics Calculations. Selective Inhibition of Oncogenic ras-p21
- Creators
- Lyndon Chie - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn, New York 11209, USA.James Chen - Computational Chemistry Division, Wyeth-Ayerst Corporation, Pearl River,Fred Friedman - Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda,Denise Chung - Long Island UniversityShazia Amar - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn,Josef Michl - SUNY Downstate Health Sciences UniversityZ Yamaizumi - National Cancer Institute, Tokyo, JapanPaul Brandt-rauf - Royal College of PhysiciansMatthew Pincus - Department of Pathology and Laboratory Medicine, Harbor VA Medical Center, Brooklyn
- Publication Details
- Journal of protein chemistry, v 18(8), pp 875-879
- Publisher
- Springer Nature B.V
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Drexel University
- Web of Science ID
- WOS:000087092800008
- Scopus ID
- 2-s2.0-53149085963
- Other Identifier
- 991019323668704721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology