Journal article
Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab
Journal of clinical microbiology, v 32(4), pp 1054-1057
Apr 1994
PMID: 8027309
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.
Metrics
Details
- Title
- Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab
- Creators
- R M Wadowsky - Department of Pathology, University of Pittsburgh, Pennsylvania 15261S Laus - Department of Pathology, University of Pittsburgh, Pennsylvania 15261T Libert - Department of Pathology, University of Pittsburgh, Pennsylvania 15261S J States - Department of Pathology, University of Pittsburgh, Pennsylvania 15261G D Ehrlich - Department of Pathology, University of Pittsburgh, Pennsylvania 15261
- Publication Details
- Journal of clinical microbiology, v 32(4), pp 1054-1057
- Publisher
- American Society for Microbiology (ASM)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1994NB46900036
- Scopus ID
- 2-s2.0-0028288881
- Other Identifier
- 991014878242304721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Microbiology