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Inhibition of cellular alpha-glucosidases results in increased presentation of hepatitis B virus glycoprotein-derived peptides by MHC class I
Journal article   Open access   Peer reviewed

Inhibition of cellular alpha-glucosidases results in increased presentation of hepatitis B virus glycoprotein-derived peptides by MHC class I

Ender Simsek, Gomathinayagam Sinnathamby, Timothy M Block, Yuanjie Liu, Ramila Philip, Anand S Mehta and Pamela A Norton
Virology (New York, N.Y.), v 384(1)
2009
PMID: 19091367
url
https://doi.org/10.1016/j.virol.2008.11.027View
Published, Version of Record (VoR) Open

Abstract

HBV envelope glycoprotein Cytotoxic T lymphocytes Glucosidase inhibitor
Inhibitors of alpha glucosidases prevent the trimming of oligosaccharides on certain nascent glycoproteins, including the hepatitis B virus MHBs envelope glycoprotein. MHBs proteins with untrimmed oligosaccharides do not interact with calnexin, increasing protein misfolding and subsequent degradation by proteasomes. As peptides loaded onto newly synthesized MHC class I complexes are predominantly derived from proteasomes, the possibility that glucosidase inhibition could increase presentation by MHC class I was determined. Using either a model epitope, or a natural MHBs epitope, it was demonstrated that glucosidase inhibitors enhanced presentation by MHC class I and promoted activation of antigen-specific CTLs, suggesting a pharmacologic approach to immune modulation.

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Virology
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