Journal article
Inhibition of microtubule nucleation at the neuronal centrosome compromises axon growth
Neuron (Cambridge, Mass.), v 12(2), pp 271-280
1994
PMID: 8110458
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We tested the dependence of axon growth on microtubule (MT) nucleation from the neuronal centrosome. Nocodazole diminished MTs in freshly plated neurons by >99%. Within 5 min of drug removal, MTs reassembled at the centrosome. This response was inhibited in cells microinjected with γ-tubulin antibody. Within 2 hr of drug removal, uninjected neurons grew >500 μm of axon. In roughly half of the antibody-injected cells, axon growth was abolished and MT levels were reduced by 8̃7% compared with uninjected cells. In the other antibody-injected cells, axon growth was compromised but not abolished, and MT levels were reduced by 3̃8%. Thus inhibition of MT nucleation at the centrosome hindered MT reassembly, and depending on the severity of this response, axon growth was either compromised or abolished.
Metrics
Details
- Title
- Inhibition of microtubule nucleation at the neuronal centrosome compromises axon growth
- Creators
- Fridoon J Ahmad - Department of Anatomy The University of Wisconsin Medical School Madison, Wisconsin 53706, USAHarish C Joshi - Department of Anatomy and Cell Biology Emory University School of Medicine Atlanta, Georgia 30322, USAVictoria E Centonze - Integrated Microscopy Resource The University of Wisconsin-Madison Madison, Wisconsin 53706, USAPeter W Baas - Department of Anatomy The University of Wisconsin Medical School Madison, Wisconsin 53706, USA
- Publication Details
- Neuron (Cambridge, Mass.), v 12(2), pp 271-280
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:A1994MY50000004
- Scopus ID
- 2-s2.0-0028324308
- Other Identifier
- 991014878530204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Neurosciences