Journal article
Inhibition of the proteasomal function in chondrocytes down-regulates growth plate chondrogenesis and longitudinal bone growth
Endocrinology (Philadelphia), v 147(8), pp 3761-3768
01 Aug 2006
PMID: 16675523
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The proteasome is a large multiprotein complex that processes intracellular proteins functioning as cell cycle regulators and transcription factors. It has been shown that the chymotryptic component of the proteasome is an important regulator of osteoblast differentiation and bone formation, with inhibitors of the proteasome increasing osteoblast differentiation and bone formation. Yet, little is known about the effects of the proteasomal activity in the growth plate. In the present study, we cultured rat metatarsal bones in the presence of proteasome inhibitor I (PSI), a known inhibitor of the chymotrypsin-like activity of the 20S proteasome. PSI suppressed growth plate chondrocyte proliferation and hypertrophy/differentiation, and induced chondrocyte apoptosis. All these cellular effects led to reduced metatarsal linear growth. In cultured chondrocytes, PSI increased the expression of beta-catenin (a negative regulator of chondrogenesis) and reduced the DNA binding of nuclear factor kappa B, a transcription factor that stimulates growth plate chondrogenesis. In conclusion, our findings suggest that the proteasomal activity facilitates growth plate chondrogenesis and, in turn, longitudinal bone growth.
Metrics
Details
- Title
- Inhibition of the proteasomal function in chondrocytes down-regulates growth plate chondrogenesis and longitudinal bone growth
- Creators
- Shufang Wu - St. Christopher's Hospital for ChildrenFrancesco De Luca - St. Christopher's Hospital for ChildrenFrancesco DeLuca - Pediatrics
- Publication Details
- Endocrinology (Philadelphia), v 147(8), pp 3761-3768
- Publisher
- Endocrine Soc
- Number of pages
- 8
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine
- Web of Science ID
- WOS:000239074100015
- Scopus ID
- 2-s2.0-33746061645
- Other Identifier
- 991019169789604721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Endocrinology & Metabolism