Logo image
Inner filter effects and their interferences in the interpretation of culture fluorescence
Journal article   Peer reviewed

Inner filter effects and their interferences in the interpretation of culture fluorescence

S P Srinivas and R Mutharasan
Biotechnology and bioengineering, v 30(6), pp 769-774
20 Oct 1987
PMID: 18581495

Abstract

Inner filter effects and their interferences in the measurement and interpretation of culture fluorescence are discussed. An approximate light intensity model for a typical open-ended culture fluorescence measuring device is developed for calculating the fluorescence response of a component of interest in a general three component solution. The model is tested using well defined synthetic fluorescent systems. The model is then extended for correlating culture fluorescence with cell density and metabolic state of microbial cultures based on a lumping approximation. The extended model has been utilized to derive culture fluorescence parameters of yeast culture at three distinct metabolic states.

Metrics

4 Record Views
60 citations in Scopus

Details

UN Sustainable Development Goals (SDGs)

This publication has contributed to the advancement of the following goals:

#3 Good Health and Well-Being

InCites Highlights

Data related to this publication, from InCites Benchmarking & Analytics tool:

Web of Science research areas
Biotechnology & Applied Microbiology
Logo image