Journal article - Review
Innovative approach in malaria research: Harnessing CRISPR-Cas9 for antimalarial drug-resistance studies in Africa
Molecular therapy, v 34(4), pp 1909-1920
01 Apr 2026
PMID: 41472363
Featured in Collection : Drexel's Newest Publications
Abstract
Malaria remains a major global health challenge, particularly in sub-Saharan Africa, where Plasmodium falciparum is increasingly resistant to frontline antimalarial therapies. CRISPR-Cas9, a groundbreaking genome editing tool, has become a transformative force in biomedical research, offering valuable insights into the genetic mechanisms underlying drug resistance. This review provides a comprehensive overview of the CRISPR-Cas9 system, its origin, evolution, and application in antimalarial drug-resistance research, with particular emphasis on African studies. We examine the role of CRISPR in elucidating resistance pathways, validating molecular markers, and enhancing diagnostic strategies. Additionally, a systematic scoping review highlights the geographic scope and focus of CRISPR-related malaria research conducted across Africa. Significantly low application and adoption were observed. Despite existing technological and regulatory barriers, CRISPR holds strong potential for accelerating antimalarial drug discovery and advancing precision medicine. Its strategic application in malaria-endemic regions could significantly bolster efforts toward disease control and eventual elimination.
CRISPR-Cas9 is transforming research on Plasmodium falciparum drug resistance in Africa. This review summarizes its use in identifying resistance mechanisms and validating molecular markers. Despite limited uptake, CRISPR offers a promising approach to guide the development of antimalarial drugs and inform strategies for malaria control in endemic regions.
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Details
- Title
- Innovative approach in malaria research: Harnessing CRISPR-Cas9 for antimalarial drug-resistance studies in Africa
- Creators
- Olusola Ajibaye (Corresponding Author) - Nigerian Institute of Medical ResearchIdowu Samuel Dada - Nigerian Institute of Medical ResearchHaddijatou Mbye - MRC Unit the GambiaIkechukwu Chidiebere Nwankwo - Drexel University, Microbiology and ImmunologyEniyou Oriero - MRC Unit the GambiaAlfred Amambua-Ngwa - MRC Unit the Gambia
- Publication Details
- Molecular therapy, v 34(4), pp 1909-1920
- Publisher
- Elsevier Inc
- Number of pages
- 12
- Grant note
- Center for Molecular Parasitology
O.A. is grateful to receive support from the Africa Research Excellence Fund (AREF) for the Research Development Fund and the seed funds (AREF-325-OLUS-S-C0969) . Wer are grateful for the tremendous support and assistance provided by Professor Akhil Vaidya and Dr. Hangjun Ke at the Center for Molecular Parasitology, Drexel University College of Medicine, Philadelphia, Pennsylvania, in connection with this work. Ethical approval was not required for this review.
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001735319800001
- Scopus ID
- 2-s2.0-105028119422
- Other Identifier
- 991022172971704721