Journal article
Insights into the Genome of Large Sulfur Bacteria Revealed by Analysis of Single Filaments
PLoS biology, v 5(9), pp 1923-e230
Sep 2007
PMID: 17760503
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Marine sediments are frequently covered by mats of the filamentous
Beggiatoa
and other large nitrate-storing bacteria that oxidize hydrogen sulfide using either oxygen or nitrate, which they store in intracellular vacuoles. Despite their conspicuous metabolic properties and their biogeochemical importance, little is known about their genetic repertoire because of the lack of pure cultures. Here, we present a unique approach to access the genome of single filaments of
Beggiatoa
by combining whole genome amplification, pyrosequencing, and optical genome mapping. Sequence assemblies were incomplete and yielded average contig sizes of approximately 1 kb. Pathways for sulfur oxidation, nitrate and oxygen respiration, and CO
2
fixation confirm the chemolithoautotrophic physiology of
Beggiatoa
. In addition,
Beggiatoa
potentially utilize inorganic sulfur compounds and dimethyl sulfoxide as electron acceptors. We propose a mechanism of vacuolar nitrate accumulation that is linked to proton translocation by vacuolar-type ATPases. Comparative genomics indicates substantial horizontal gene transfer of storage, metabolic, and gliding capabilities between
Beggiatoa
and cyanobacteria. These capabilities enable
Beggiatoa
to overcome non-overlapping availabilities of electron donors and acceptors while gliding between oxic and sulfidic zones. The first look into the genome of these filamentous sulfur-oxidizing bacteria substantially deepens the understanding of their evolution and their contribution to sulfur and nitrogen cycling in marine sediments.
Examining the genome of single filaments of
Beggiatoa
deepens our understanding of their contribution to sulfur and nitrogen cycling in marine sediments.
Metrics
Details
- Title
- Insights into the Genome of Large Sulfur Bacteria Revealed by Analysis of Single Filaments
- Creators
- Marc Mußmann - Max Planck Institute for Marine Microbiology, Bremen, GermanyFen Z Hu - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of AmericaMichael Richter - Max Planck Institute for Marine Microbiology, Bremen, GermanyDirk de Beer - Max Planck Institute for Marine Microbiology, Bremen, GermanyAndré Preisler - Max Planck Institute for Marine Microbiology, Bremen, GermanyBo B Jørgensen - Max Planck Institute for Marine Microbiology, Bremen, GermanyMarcel Huntemann - Max Planck Institute for Marine Microbiology, Bremen, GermanyFrank Oliver Glöckner - Max Planck Institute for Marine Microbiology, Bremen, GermanyRudolf Amann - Max Planck Institute for Marine Microbiology, Bremen, GermanyWerner J. H Koopman - Department of Membrane Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The NetherlandsRoger S Lasken - J. Craig Venter Institute, Rockville, Maryland, United States of AmericaBenjamin Janto - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of AmericaJustin Hogg - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of AmericaPaul Stoodley - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of AmericaRobert Boissy - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of AmericaGarth D Ehrlich - Center for Genomic Sciences, Allegheny General Hospital/Allegheny-Singer Research Institute, Pittsburgh, Pennsylvania, United States of America
- Publication Details
- PLoS biology, v 5(9), pp 1923-e230
- Publisher
- Public Library of Science; San Francisco, USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000249552300012
- Scopus ID
- 2-s2.0-34548688408
- Other Identifier
- 991014878036104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology