Journal article
Instrument-Free Point-of-Care Molecular Detection of Zika Virus
Analytical chemistry (Washington), v 88(14), pp 7289-7294
19 Jul 2016
PMID: 27306491
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The recent outbreak of Zika virus (ZIKV) infection in the Americas and its devastating impact on fetal development have prompted the World Health Organization (WHO) to declare the ZIKV pandemic as a Public Health Emergency of International Concern. Rapid and reliable diagnostics for ZIKV are vital because ZIKV-infected individuals display no symptoms or nonspecific symptoms similar to other viral infections. Because immunoassays lack adequate sensitivity and selectivity and are unable to identify active state of infection, molecular diagnostics are an effective means to detect ZIKV soon after infection and throughout pregnancy. We report on a highly sensitive reverse-transcription loop-mediated, isothermal amplification (RT-LAMP) assay for rapid detection of ZIKV and its implementation in a simple, easy-to-use, inexpensive, point-of-care (POC) disposable cassette that carries out all the unit operations from sample introduction to detection. For thermal control of the cassette, we use a chemically heated cup without a need for electrical power. Amplification products are detected with leuco crystal violet (LCV) dye by eye without a need for instrumentation. We demonstrated the utility of our POC diagnostic system by detecting ZIKV in oral samples with sensitivity of 5 plaque-forming units (PFU) in less than 40 min. Our system is particularly suitable for resource-poor settings, where centralized laboratory facilities, funds, and trained personnel are in short supply, and for use in doctors' offices, clinics, and at home.
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Details
- Title
- Instrument-Free Point-of-Care Molecular Detection of Zika Virus
- Creators
- Jinzhao Song - University of PennsylvaniaMichael G. Mauk - Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USABrent A. Hackett - University of PennsylvaniaSara Cherry - University of PennsylvaniaHaim H. Bau - University of PennsylvaniaChangchun Liu - University of Pennsylvania
- Publication Details
- Analytical chemistry (Washington), v 88(14), pp 7289-7294
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 6
- Grant note
- K25AI099160; R21-AI-112713-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30AI045008 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) AI045008 / Penn CFAR Pilot Grant
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000380296800046
- Scopus ID
- 2-s2.0-84978792161
- Other Identifier
- 991020623911804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Analytical