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Integrated search and alignment of protein structures
Journal article   Open access   Peer reviewed

Integrated search and alignment of protein structures

Ahmet Sacan, I Hakki Toroslu and Hakan Ferhatosmanoglu
Bioinformatics (Oxford, England), v 24(24), pp 2872-2879
15 Dec 2008
PMID: 18945684
url
https://doi.org/10.1093/bioinformatics/btn545View
Published, Version of Record (VoR) Open

Abstract

Sequence Analysis, Protein Algorithms Protein Structure, Secondary Databases, Protein Models, Molecular Protein Conformation Structural Homology, Protein Proteins - chemistry
Identification and comparison of similar three-dimensional (3D) protein structures has become an even greater challenge in the face of the rapidly growing structure databases. Here, we introduce Vorometric, a new method that provides efficient search and alignment of a query protein against a database of protein structures. Voronoi contacts of the protein residues are enriched with the secondary structure information and a metric substitution matrix is developed to allow efficient indexing. The contact hits obtained from a distance-based indexing method are extended to obtain high-scoring segment pairs, which are then used to generate structural alignments. Vorometric is the first to address both search and alignment problems in the protein structure databases. The experimental results show that Vorometric is simultaneously effective in retrieving similar protein structures, producing high-quality structure alignments, and identifying cross-fold similarities. Vorometric outperforms current structure retrieval methods in search accuracy, while requiring com-parable running times. Furthermore, the structural superpositions produced are shown to have better quality and coverage, when compared with those of the popular structure alignment tools. Vorometric is available as a web service at http://bio.cse.ohio-state.edu/Vorometric

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Collaboration types
Domestic collaboration
International collaboration
Web of Science research areas
Biochemical Research Methods
Biotechnology & Applied Microbiology
Computer Science, Interdisciplinary Applications
Mathematical & Computational Biology
Statistics & Probability
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