Journal article
Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity
Molecular ecology resources, v 9(1), pp 1-26
May 2009
PMID: 21564960
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today's Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding - the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species - was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to 'variation' or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.
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Details
- Title
- Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity
- Creators
- Daniel H. Janzen - University of PennsylvaniaWinnie Hallwachs - University of PennsylvaniaPatrick Blandin - Muséum national d'Histoire naturelleJohn M. Burns - Smithsonian InstitutionJean-Marie CadiouIsidro Chacon - National Biodiversity InstituteTanya Dapkey - University of PennsylvaniaAndrew R. Deans - North Carolina State UniversityMarc E. Epstein - California Department of Food and AgricultureBernardo Espinoza - National Biodiversity InstituteJohn G. Franclemont - New York Proton CenterWilliam A. Haber - Missouri Botanical GardenMehrdad Hajibabaei - University of GuelphJason P. W. Hall - National Museum of Natural HistoryPaul D. N. Hebert - University of GuelphIan D. GauldDonald J. Harvey - National Museum of Natural HistoryAxel Hausmann - Bavarian State Collection of ZoologyIan J. Kitching - Natural History MuseumDon Lafontaine - Agriculture and Agri-Food CanadaJean-Francois Landry - Agr & Agri Food Canada, Canadian Natl Collect Insects, Ottawa, ON K1A 0C6, CanadaClaude LemaireJacqueline Y. Miller - University of FloridaJames S. Miller - Institute of EntomologyLee MillerScott E. Miller - Smithsonian InstitutionJose Montero - Inst Nacl Biodiversidad, Santo Domingo De Heredia, Costa RicaEugene MunroeSuzanne Rab Green - American Museum of Natural HistorySujeevan Ratnasingham - University of GuelphJohn E. Rawlins - Carnegie Museum of Natural HistoryRobert K. Robbins - National Museum of Natural HistoryJosephine J. Rodriguez - Goodwin CollegeRodolphe Rougerie - University of GuelphMichael J. Sharkey - University of KentuckyM. Alex Smith - University of GuelphM. Alma Solis - Smithsonian InstitutionJ. Bolling Sullivan - Technical College of the LowcountryPaul ThiaucourtDavid B. Wahl - American Entomological InstituteSusan J. Weller - University of MinnesotaJames B. Whitfield - University of MinnesotaKeith R. Willmott - Florida Museum of Natural HistoryD. Monty Wood - Agriculture and Agri-Food CanadaNorman E. Woodley - Smithsonian InstitutionJohn J. Wilson - University of Guelph
- Publication Details
- Molecular ecology resources, v 9(1), pp 1-26
- Publisher
- Wiley
- Number of pages
- 26
- Grant note
- 0809175 / Office Of Internatl Science &Engineering; Office Of The Director; National Science Foundation (NSF); NSF - Office of the Director (OD) Genome Canada through the Ontario Genomics Institute; Genome Canada Area de Conservacion Guanacaste Guanacaste Dry Forest Conservation Fund Wege Foundation; ACEV Foundation Science Connection NSERC; Natural Sciences and Engineering Research Council of Canada (NSERC) Jessie B. Cox Charitable Trust INBio BSR 9024770; DEB 9306296; 9400829; 9705072; 0072730; 0515699 / US National Science Foundation; National Science Foundation (NSF)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Academy of Natural Sciences of Drexel University
- Web of Science ID
- WOS:000265227700001
- Scopus ID
- 2-s2.0-65349140121
- Other Identifier
- 991022048272304721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Ecology
- Evolutionary Biology