Journal article
Interaction Of HTLV-1 Tax With The Proteins Of Cellular Secretory Pathway
AIDS research and human retroviruses, Vol.21(5), pp.511-511
01 May 2005
Abstract
Background: Tax protein has been shown to play an integral role in HTLV-I-induced disease pathogenesis. Extracellular Tax has been detected in the cerebrospinal fluid of HAM/TSP patients, suggesting that cell-free Tax may be physiologically involved in the progression of neurologic disease. We have previously demonstrated the secretion of full length Tax and its co-localization with the cytoplasmic organelles relevant to secretion. Methods: The present study elucidates the mechanism of Tax secretion. To identify Tax interacting proteins within cell, we have used an antibody array spotted with antibodies against cellular secretory pathway proteins. Upon hybridization with protein extracts from Tax-treated cells, antibody array analyses have suggested the interaction of Tax with SCAMP1, SCAMP2, SNAP23, and COPII; proteins that facilitate transport between nucleus and cytoplasm, and between endoplasmic reticulum, Golgi complex, and plasma membrane. Results: Subsequently, we have confirmed these specific protein-protein interactions by co-immunoprecipitation and GST pull-down assay involving the use of GST-Tax fusion protein bound to glutathione-coupled particles to affinity purify any proteins that interact with Tax. Conclusions: Collectively, these studies have demonstrated the interaction of Tax with multiple proteins in the secretory pathway and that Tax may be secreted and act as an extracellular effector molecule.
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Details
- Title
- Interaction Of HTLV-1 Tax With The Proteins Of Cellular Secretory Pathway
- Creators
- K FlaigP JainB Wigdahl
- Publication Details
- AIDS research and human retroviruses, Vol.21(5), pp.511-511
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170497804721