Journal article
Interaction between human T cell leukemia virus type 1 Tax and protein components of the cellular secretory pathway
Journal of neurovirology, Vol.12, pp.27-27
01 May 2006
Abstract
Tax protein has been shown to play an integral role in human T cell leukemia virus-1-induced diseases including adult T cell leukemia (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Extracellular Tax has been detected in the cerebrospinal fluid of HAM/TSP patients, suggesting that cell-free Tax may be physiologically involved in the progression of neurologic disease. We have previously demonstrated the secretion of full-length Tax and its co-localization with the cytoplasmic organelles relevant to secretion. The present study elucidates the mechanism of Tax secretion. To identify intracellular Tax interacting proteins, we have used an antibody array spotted with antibodies directed against cellular secretory pathway proteins. Upon reaction with protein extracts from Tax-treated cells, antibody array analyses have suggested the interaction of Tax with SCAMP1, SCAMP2, SNAP23, and COPII; proteins that facilitate transport between the nucleus and cytoplasm, and between the endoplasmic reticulum, Golgi complex, and plasma membrane. Subsequently, we have confirmed these specific protein-protein interactions by co-immunoprecipitation and GST pull-down assay involving the use of a GST-Tax fusion protein bound to glutathione-coupled particles to affinity purify proteins that interact with Tax. Collectively, these studies have demonstrated the interaction of Tax with multiple proteins in the secretory pathway and that Tax may be secreted and act as an extracellular effector molecule.
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Details
- Title
- Interaction between human T cell leukemia virus type 1 Tax and protein components of the cellular secretory pathway
- Creators
- K E FlaigP JainK MostollerD PandyaB Wigdahl
- Publication Details
- Journal of neurovirology, Vol.12, pp.27-27
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170469304721