Journal article
Interaction of apolipoprotein A-I with lecithin-cholesterol vesicles in the presence of phospholipase C
Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1635(2)
2003
PMID: 14729075
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Here we study the anti-nucleating mechanism of apolipoprotein A-I (apo A-I) on model biliary vesicles in the presence of phospholipase C (PLC) utilizing dynamic light scattering (DLS), steady-state fluorescence spectroscopy, cryogenic transmission electron microscopy (cryo-TEM), and UV/Vis spectroscopy. PLC induces aggregation of cholesterol-free lecithin vesicles from an initial, average size of 100 nm to a maximal size of 600 nm. The presence of apo A-I likely inhibits vesicle aggregation by shielding the PLC-generated hydrophobic moieties, which results in vesicles of an average size of 200 nm. A similar phenomenon is observed in cholesterol-enriched lecithin vesicles. Whereas PLC alone produces aggregates of 300 nm, no aggregation is observed when apo A-I is present along with PLC. However, the ability of apo A-I to inhibit aggregation is temporary, and after 8 h, a broad particle size distribution with sizes as high as 800 nm is observed. Apo A-I possibly induces the formation of small apo A-I/lecithin/cholesterol complexes of about 5–20 nm similar to the discoidal pre-HDL complexes found in blood when it can no longer effectively shield all the DAG molecules. Concomitant with formation of complexes, DAG molecules coalesce into large oil droplets, which account for the large particles observed by light scattering. Thus, apo A-I acts as an anti-nucleating agent by two mechanisms, anti-aggregation and microstructural transition. The mode of protection is dependent on the cholesterol content and the relative amounts of DAG and apo A-I present. This study supports the possibility of apo A-I solubilizing lipids in bile in a similar fashion as it does in blood and also delineates the mechanism of formation of the complexes.
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Details
- Title
- Interaction of apolipoprotein A-I with lecithin-cholesterol vesicles in the presence of phospholipase C
- Creators
- Manasa V Gudheti - Department of Chemical Engineering, College of Engineering, Drexel University, 32nd & Chestnut Streets, Philadelphia, PA 19104, USAYamaira I Gonzalez - Center for Molecular and Engineering Thermodynamics, University of Delaware, Newark, DE 19716, USASum P Lee - School of Medicine, University of Washington, Seattle, WA 98195, USASteven P Wrenn - Department of Chemical Engineering, College of Engineering, Drexel University, 32nd & Chestnut Streets, Philadelphia, PA 19104, USA
- Publication Details
- Biochimica et biophysica acta. Molecular and cell biology of lipids, v 1635(2)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000188511000008
- Scopus ID
- 2-s2.0-0346056939
- Other Identifier
- 991014878619404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- Cell Biology