Journal article
Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast
Genes to cells : devoted to molecular & cellular mechanisms, v 14(6), pp 669-682
01 Jun 2009
PMID: 19422421
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The Swi1-Swi3 replication fork protection complex and Mrc1 protein are required for stabilization of stalled replication forks in fission yeast. Hsk1 kinase also plays roles in checkpoint responses elicited by arrested replication forks. We show that both Swi1 and Swi3, the abundance of which are interdependent, are required for chromatin association of Mrc1. Co-immunoprecipitation experiments show the interactions of Swi1-Swi3, Mrc1 and Hsk1. Mrc1 interacts with Swi3 and Hsk1 proteins through its central segment (378-879) containing a SQ/TQ cluster, and this segment is sufficient for checkpoint reaction. The SQ/TQ cluster segment (536-673) is essential but not sufficient for the interactions and for resistance to replication inhibitor hydroxyurea. Mrc1 protein level is increased in hsk1-89 cells due to apparent stabilization, and we have identified a potential phosphodegron sequence. These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast.
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Details
- Title
- Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast
- Creators
- Michie Shimmoto - Institute of Medical ScienceSeiji Matsumoto - Institute of Medical ScienceYukari Odagiri - Institute of Medical ScienceEishi Noguchi - Drexel UniversityPaul Russell - Scripps Research InstituteHisao Masai - Institute of Medical Science
- Publication Details
- Genes to cells : devoted to molecular & cellular mechanisms, v 14(6), pp 669-682
- Publisher
- Wiley
- Number of pages
- 14
- Grant note
- Ministry of Education, Culture, Sports, Science and Technology of Japan; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) R01GM077604 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) GM077604 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000266701800001
- Scopus ID
- 2-s2.0-66749154726
- Other Identifier
- 991019169638804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Cell Biology
- Genetics & Heredity