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Interactive image analysis programs for quantifying injury-induced axonal beading and microtubule disruption
Journal article   Open access   Peer reviewed

Interactive image analysis programs for quantifying injury-induced axonal beading and microtubule disruption

Devrim Kilinc, Gianluca Gallo and Kenneth A Barbee
Computer methods and programs in biomedicine, v 95(1), pp 62-71
2009
PMID: 19285748
url
https://doi.org/10.1016/j.cmpb.2009.01.002View
Published, Version of Record (VoR)Maybe Open Access (Publisher Bronze) Open

Abstract

Image analysis Axonal beading Axonal injury Morphometric analysis
Focal axonal beading and focal disruption of microtubule structure are characteristic to traumatic axonal injury. We have recently reproduced these morphological and structural changes in our in vitro model system [D. Kilinc, G. Gallo, K.A. Barbee, Mechanically induced membrane poration causes axonal beading and localized cytoskeletal damage, Exp. Neurol. 212 (2008) 422–430]. In order to measure bead formation objectively, an observer-independent quantification of beading was necessary. In addition, a quantitative measure for the extent of co-localization of axonal beads and microtubule disruptions was required to establish a causal relationship between focal cytoskeletal damage and bead formation. In this paper we describe Matlab-based, interactive image analysis programs for axonal beading quantification and co-localization analysis. Injury-induced increases in the axonal beading could be successfully detected using the bead analysis program.

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Web of Science research areas
Computer Science, Interdisciplinary Applications
Computer Science, Theory & Methods
Engineering, Biomedical
Medical Informatics
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