Journal article
Interactive image analysis programs for quantifying injury-induced axonal beading and microtubule disruption
Computer methods and programs in biomedicine, Vol.95(1), pp.62-71
2009
PMID: 19285748
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Focal axonal beading and focal disruption of microtubule structure are characteristic to traumatic axonal injury. We have recently reproduced these morphological and structural changes in our
in vitro model system [D. Kilinc, G. Gallo, K.A. Barbee, Mechanically induced membrane poration causes axonal beading and localized cytoskeletal damage, Exp. Neurol. 212 (2008) 422–430]. In order to measure bead formation objectively, an observer-independent quantification of beading was necessary. In addition, a quantitative measure for the extent of co-localization of axonal beads and microtubule disruptions was required to establish a causal relationship between focal cytoskeletal damage and bead formation. In this paper we describe Matlab-based, interactive image analysis programs for axonal beading quantification and co-localization analysis. Injury-induced increases in the axonal beading could be successfully detected using the bead analysis program.
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Details
- Title
- Interactive image analysis programs for quantifying injury-induced axonal beading and microtubule disruption
- Creators
- Devrim Kilinc - School of Biomedical Engineering, Science, and Health Systems, Drexel University, 3141 Chestnut St., Philadelphia, PA 19104, USAGianluca Gallo - Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129, USAKenneth A Barbee - School of Biomedical Engineering, Science, and Health Systems, Drexel University, 3141 Chestnut St., Philadelphia, PA 19104, USA
- Publication Details
- Computer methods and programs in biomedicine, Vol.95(1), pp.62-71
- Publisher
- Elsevier Ireland Ltd
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Identifiers
- 991014878428904721
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