Journal article
Interferon-γ and Interleukin-10 Reciprocally Regulate Endothelial Junction Integrity and Barrier Function
Microvascular research, v 61(1), pp 130-143
Jan 2001
PMID: 11162203
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Inflammatory bowel disease (IBD) is associated with Th1/Th2 cytokine dysregulation, leukocyte extravasation, and tissue edema, but the mechanisms for cytokine-mediated vascular dysfunction are not understood. To investigate how cytokines might control edema in IBD, we determined vascular permeability and IFN-γ expression in two models of murine colitis: SCID mice reconstituted with CD45RBhigh T-lymphocytes (CD45RBhigh/SCID mice), and interleukin-10 gene deficient (IL-10−/−) mice. We also investigated the in vitro effects of IFN-γ and IL-10 on human endothelial solute barrier and junction protein expression. Vascular permeability in CD45RBhigh/SCID and IL-10−/− mice was quantified using tissue 131I-IgG accumulation. The IFN-γ message was quantified using the ribonuclease protection assay. Endothelial barrier integrity in vitro was measured by transmonolayer electrical resistance, and junctional proteins were examined by immunoblotting and fluorescence microscopy. Both CD45RBhigh/SCID and IL-10−/− mice exhibit enhanced colonic microvascular leakage and IFN-γ message levels compared to their respective controls. In vitro, IFN-γ also reduced endothelial barrier (monolayer electrical resistance, increased albumin permeability) and reduced tight junction (occludin) expression and staining. These effects were reversed by pretreatment of monolayers with IL-10. Therefore, in vivo IFN-γ and IL-10 may modulate microvascular leakage in IBD partly by controlling the expression of intestinal endothelial tight junctional proteins.
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Details
- Title
- Interferon-γ and Interleukin-10 Reciprocally Regulate Endothelial Junction Integrity and Barrier Function
- Creators
- Tadayuki Oshima - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932F.Stephen Laroux - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Laura L Coe - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Zenichi Morise - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Shigeyuki Kawachi - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Philippe Bauer - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Matthew B Grisham - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Robert D Specian - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Patsy Carter - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Stephen Jennings - Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932D.Neil Granger - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932Takashi Joh - First Department of Internal Medicine, Nagoya City University Medical School, Nagoya, 467-8601, JapanJ.Steven Alexander - Department of Molecular and Cellular Physiology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, 71130-3932
- Publication Details
- Microvascular research, v 61(1), pp 130-143
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000166821900013
- Scopus ID
- 2-s2.0-0034757324
- Other Identifier
- 991014878043704721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Peripheral Vascular Disease