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Interleukin-15 Increases Effector Memory CD8+ T Cells and NK Cells in Simian Immunodeficiency Virus-Infected Macaques
Journal article   Open access   Peer reviewed

Interleukin-15 Increases Effector Memory CD8+ T Cells and NK Cells in Simian Immunodeficiency Virus-Infected Macaques

Yvonne M Mueller, Constantinos Petrovas, Paul M Bojczuk, Ioannis D Dimitriou, Brigitte Beer, Peter Silvera, Francois Villinger, J. Scott Cairns, Edward J Gracely, Mark G Lewis, …
Journal of virology, v 79(8), pp 4877-4885
Apr 2005
PMID: 15795273
url
https://doi.org/10.1128/JVI.79.8.4877-4885.2005View
Published, Version of Record (VoR) Open

Abstract

Cellular Response to Infection
Interleukin-15 (IL-15) in vitro treatment of peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus (HIV)-infected individuals specifically enhances the function and survival of HIV-specific CD8 + T cells, while in vivo IL-15 treatment of mice preferentially expands memory CD8 + T cells. In this study, we investigated the in vivo effect of IL-15 treatment in 9 SIVmac251-infected cynomolgus macaques (low dose of IL-15, 10 μg/kg of body weight, n = 3; high dose of IL-15, 100 μg/kg, n = 3; control [saline], n = 3; dose administered twice weekly for 4 weeks). IL-15 treatment induced a nearly threefold increase in peripheral blood CD8 + CD3 − NK cells. Furthermore, CD8 + T-cell numbers increased more than twofold, mainly due to an increase in the CD45RA − CD62L − and CD45RA + CD62L − effector memory CD8 + T cells. Expression of Ki-67 in the CD8 + T cells indicated expansion of CD8 + T cells and not redistribution. IL-15 did not affect CD4 + T-cell, B-cell, and CD14 + macrophage numbers. No statistically significant differences in changes from baseline in the viral load were observed when control-, low-dose-, and high-dose-treated animals were compared. No clinical adverse effects were observed in any of the animals studied. The selective expansion of effector memory CD8 + T cells and NK cells by IL-15 further supports IL-15's possible therapeutic use in viral infections such as HIV infection.

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Virology
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