Journal article
Interplay of dendritic cell surface receptors and adhesion molecules with respect to HTLV-1 binding, entry, and transmission
Journal of neurovirology, Vol.13, pp.100-100
01 Jan 2007
Abstract
Despite the susceptibility of dendritic cells (DCs) to HTLV-1 infection and a demonstrated role of these cells in disease pathogenesis, the mechanisms of virus binding and entry have not been delineated. The identification of HTLV-1 receptor has been hindered due to its ubiquitous nature. Recently, a glucose transporter GLUT-1, heparan sulfate proteoglycans (HSPGs) and Neuropilin-1 (NRP-1) were demonstrated to facilitate HTLV-1 binding and entry, primarily in T cells. DCs express their own array of antigen receptors, most notably DC-specific ICAM-3-grabbing non-integrin (DC-SIGN), with respect to retrovirus binding. In this study, we analyzed the role of DC-SIGN and other known HTLV-1 receptors with respect to viral binding, entry, and transmission using primary human monocyte-derived DCs and B cell lines stably expressing DC-SIGN. A comparative expression of DC-SIGN, GLUT-1, HSPG, and NRP-1 was examined and correlated with a number of biological endpoints. Inhibition of all these molecules dramatically reduced the binding of HTLV-1 virions as well as envelope glycoprotein gp46. With respect to HTLV-1 entry, GLUT-1 and HSPCs played an important role while inhibition of DC-SIGN and NP-1 had no apparent effect. Finally, the transmission of HTLV-1 from infected DCs to target T cells was analyzed and was found to be primarily mediated by DC-SIGN consistent with its known role for other viruses. These observations were also repeated ex vivo with myeloid and plasmacytoid DCs. This study represents the first comprehensive analyses of known HTLV-1 receptors on a major immune cell population that plays a critical role in viral binding and transmission.
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Details
- Title
- Interplay of dendritic cell surface receptors and adhesion molecules with respect to HTLV-1 binding, entry, and transmission
- Creators
- S ManuelB WigdahlJ AhujaZ KhanP Jain
- Publication Details
- Journal of neurovirology, Vol.13, pp.100-100
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170495004721