Journal article
Into the Wild: A novel wild-derived inbred strain resource expands the genomic and phenotypic diversity of laboratory mouse models
PLoS genetics, v 20(4), pp e1011228-e1011228
10 Apr 2024
PMID: 38598567
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The laboratory mouse has served as the premier animal model system for both basic and preclinical investigations for over a century. However, laboratory mice capture only a subset of the genetic variation found in wild mouse populations, ultimately limiting the potential of classical inbred strains to uncover phenotype-associated variants and pathways. Wild mouse populations are reservoirs of genetic diversity that could facilitate the discovery of new functional and disease-associated alleles, but the scarcity of commercially available, well-characterized wild mouse strains limits their broader adoption in biomedical research. To overcome this barrier, we have recently developed, sequenced, and phenotyped a set of 11 inbred strains derived from wild-caught Mus musculus domesticus. Each of these "Nachman strains" immortalizes a unique wild haplotype sampled from one of five environmentally distinct locations across North and South America. Whole genome sequence analysis reveals that each strain carries between 4.73-6.54 million single nucleotide differences relative to the GRCm39 mouse reference, with 42.5% of variants in the Nachman strain genomes absent from current classical inbred mouse strain panels. We phenotyped the Nachman strains on a customized pipeline to assess the scope of disease-relevant neurobehavioral, biochemical, physiological, metabolic, and morphological trait variation. The Nachman strains exhibit significant inter-strain variation in >90% of 1119 surveyed traits and expand the range of phenotypic diversity captured in classical inbred strain panels. These novel wild-derived inbred mouse strain resources are set to empower new discoveries in both basic and preclinical research.
Metrics
Details
- Title
- Into the Wild: A novel wild-derived inbred strain resource expands the genomic and phenotypic diversity of laboratory mouse models
- Creators
- Beth L Dumont - University of MaineDaniel M Gatti - Jackson LaboratoryMallory A Ballinger - Cornell UniversityDana Lin - Vanderbilt UniversityMegan Phifer-Rixey - Department of Biology, Drexel University, Philadelphia, Pennsylvania, United States of AmericaMichael J Sheehan - Cornell UniversityTaichi A Suzuki - Arizona State UniversityLydia K Wooldridge - Jackson LaboratoryHilda Opoku Frempong - University of MaineRaman Akinyanju Lawal - Jackson LaboratoryGary A Churchill - Jackson LaboratoryCathleen Lutz - Jackson LaboratoryNadia Rosenthal - Jackson LaboratoryJacqueline K White - Jackson LaboratoryMichael W Nachman - Museum of Vertebrate Zoology
- Publication Details
- PLoS genetics, v 20(4), pp e1011228-e1011228
- Publisher
- Public LIbrary of Science (PLOS)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:001202796000001
- Scopus ID
- 2-s2.0-85190333886
- Other Identifier
- 991021868015704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Genetics & Heredity