Journal article
Intracerebral microdialysis study of glutamate reuptake in awake, behaving rats
Brain research, v 766(1)
1997
PMID: 9359581
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The central nervous system has high-affinity uptake systems for the clearance of amino acid transmitters. These systems are found in both neurons and astrocytes. Previous studies have shown that the uptake of amino acid transmitters by astrocytes in culture can be modulated by adrenergic agents. The objectives of this study were to develop a methodology that evaluates the brain's reuptake capacity for glutamate in awake, behaving animals and to determine whether glutamate reuptake is under α-adrenergic regulation in the intact central nervous system. Male Sprague–Dawley rats weighing 250–450 g were used in this study. The extraction fraction of
l-[
3H]glutamate with [
14C]mannitol as a reference was measured. The cortical extraction fraction of
l-[
3H]glutamate corrected for [
14C]mannitol (
E
l-glu
) reaches steady state rapidly and is both stable and repeatable.
E
l-glu
is a measure of
l-glutamate reuptake and not metabolism.
E
l-glu
is decreased in a dose-dependent manner by the addition of the glutamate reuptake blocker
d,
l-threo-β-hydroxyaspartic acid or unlabeled
l- glutamate. In addition,
E
l-glu
is increased in a dose-dependent manner by the α
1-adrenergic agonist phenylephrine, and this increase is blocked by the α-adrenergic antagonist phentolamine.
Metrics
Details
- Title
- Intracerebral microdialysis study of glutamate reuptake in awake, behaving rats
- Creators
- Guillermo M. Alexander - Allegheny University of the Health SciencesJohn R. Grothusen - Allegheny University of the Health SciencesSharon W. Gordon - Allegheny University of the Health SciencesRobert J. Schwartzman - Allegheny University of the Health Sciences
- Publication Details
- Brain research, v 766(1)
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- [Retired Faculty]; Neurology
- Web of Science ID
- WOS:A1997XX88900001
- Scopus ID
- 2-s2.0-0030886112
- Other Identifier
- 991019168810604721
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InCites Highlights
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- Web of Science research areas
- Neurosciences