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Intracisternal rSV40 administration provides effective pan-CNS transgene expression
Journal article   Peer reviewed

Intracisternal rSV40 administration provides effective pan-CNS transgene expression

J-P Louboutin, B. A. S. Reyes, L. Agrawal, E. J. Van Bockstaele and D. S. Strayer
Gene therapy, v 19(1), pp 114-118
01 Jan 2012
PMID: 21614027
url
https://doi.org/10.1038/gt.2011.75View
Published, Version of Record (VoR) Restricted

Abstract

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Genetics & Heredity Life Sciences & Biomedicine Medicine, Research & Experimental Research & Experimental Medicine Science & Technology
Potential genetic treatments for many generalized central nervous system (CNS) diseases require transgene expression throughout the CNS. Using oxidant stress and apoptosis caused by HIV-1 envelope gp120 as a model, we studied pan-CNS neuroprotective gene delivery into the cisterna magna (CM). Recombinant SV40 vectors carrying Cu/Zn superoxide dismutase or glutathione peroxidase were injected into rat CMs following intraperitoneal administration of mannitol. Sustained transgene expression was seen in neurons throughout the CNS. On challenge, 8 weeks later with gp120 injected into the caudate putamen, significant neuroprotection was documented. Thus, intracisternal administration of antioxidant-carrying rSV40 vectors may be useful in treating widespread CNS diseases such as HIV-1-associated neurocognitive disorders characterized by oxidative stress. Gene Therapy (2012) 19, 114-118; doi:10.1038/gt.2011.75; published online 26 May 2011

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Web of Science research areas
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Genetics & Heredity
Medicine, Research & Experimental
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