Journal article
Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages
Immunobiology (1979), v 216(11), pp 1164-1171
01 Nov 2011
PMID: 21802164
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Uropathogenic E. coli (UPEC) are the primary cause of urinary tract infections. Recent studies have demonstrated that UPEC can invade and replicate within epithelial cells, suggesting that this bacterial pathogen may occupy an intracellular niche within the host. Given that many intracellular pathogens target macrophages, we assessed the interactions between UPEC and macrophage's. Colonization of the mouse bladder by UPEC strain CFT073 resulted in increased expression of myeloid-restricted genes, consistent with the recruitment of inflammatory macrophages to the site of infection. In in vitro assays, CFT073 was able to survive within primary mouse bone marrow-derived macrophages (BMM) up to 24 h post-infection. Three additional well-characterized clinical UPEC isolates associated with distinct UTI symptomatologies displayed variable long-term survival within BMM. UPEC strains UTI89 and VR50, originally isolated from patients with cystitis and asymptomatic bacteriuria respectively, showed elevated bacterial loads in BMM at 24 h post-infection as compared to CFT073 and the asymptomatic bacteriuria strain 83972. These differences did not correlate with differential effects on macrophage survival or initial uptake of bacteria. E. con UTI89 localized to a Lamp1(+) vesicular compartment within BMM. In contrast to survival within mouse BMM, intracellular bacterial loads of VR50 were low in both human monocyte-derived macrophages (HMDM) and in human T24 bladder epithelial cells. Collectively, these data suggest that some UPEC isolates may subvert macrophage anti-microbial pathways, and that host species differences may impact on intracellular UPEC survival. (C) 2011 Elsevier GmbH. All rights reserved.
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Details
- Title
- Intramacrophage survival of uropathogenic Escherichia coli: Differences between diverse clinical isolates and between mouse and human macrophages
- Creators
- Nilesh J. Bokil - The University of QueenslandMakrina Totsika - The University of QueenslandAlison J. Carey - Griffith UniversityKatryn J. Stacey - The University of QueenslandViktoria Hancock - Technical University of DenmarkBernadette M. Saunders - Centenary InstituteTimothy Ravasi - King Abdullah University of Science and TechnologyGlen C. Ulett - Griffith UniversityMark A. Schembri - The University of QueenslandMatthew J. Sweet - The University of Queensland
- Publication Details
- Immunobiology (1979), v 216(11), pp 1164-1171
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- ARC; Australian Research Council APP1005315; 631531; 569674 / National Health and Medical Research Council of Australia; National Health and Medical Research Council (NHMRC) of Australia NHMRC; National Health and Medical Research Council (NHMRC) of Australia ACRF
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pediatrics; College of Medicine; Drexel University
- Web of Science ID
- WOS:000296269700002
- Scopus ID
- 2-s2.0-80053236888
- Other Identifier
- 991020099678904721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Immunology