Journal article
Intraspinal microinjection of chondroitinase ABC following injury promotes axonal regeneration out of a peripheral nerve graft bridge
Experimental neurology, v 211(1)
May 2008
PMID: 18353313
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Chondroitin sulfate proteoglycans (CSPG) within the glial scar formed after central nervous system (CNS) injury are thought to play a crucial role in regenerative failure. We previously showed that delivery of the CSPG-digesting enzyme chondroitinase ABC (ChABC) via an osmotic minipump allowed axonal regeneration and functional recovery in a peripheral nerve graft (PNG)-bridging model. In this study, we sought to overcome the technical limitations associated with minipumps by microinjecting ChABC directly into the distal lesion site in the PN bridging model. Microinjection of ChABC immediately rostral and caudal to an injury site resulted in extensive CSPG digestion. We also demonstrate that this delivery technique is relatively atraumatic and does not result in a noticeable inflammatory response. Importantly, microinjections of ChABC into the lesion site permitted more regenerating axons to exit a PNG and reenter spinal cord tissue than saline injections. These results are similar to our previous findings when ChABC was delivered via a minipump and suggest that microinjecting ChABC is an effective method of delivering the potentially therapeutic enzyme directly to an injury site.
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Details
- Title
- Intraspinal microinjection of chondroitinase ABC following injury promotes axonal regeneration out of a peripheral nerve graft bridge
- Creators
- Veronica J Tom - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 2900 Queen Lane, Philadelphia, PA 19129, USAJohn D Houlé
- Publication Details
- Experimental neurology, v 211(1)
- Publisher
- Elsevier; United States
- Grant note
- R37 NS026380-21 / NINDS NIH HHS NS 26380 / NINDS NIH HHS R37 NS026380 / NINDS NIH HHS R01 NS026380 / NINDS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000255725600033
- Scopus ID
- 2-s2.0-42749092831
- Other Identifier
- 991014877758804721
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- Web of Science research areas
- Neurosciences