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Intrinsic Role of FoxO3a in the Development of CD8(+) T Cell Memory
Journal article   Open access   Peer reviewed

Intrinsic Role of FoxO3a in the Development of CD8(+) T Cell Memory

Fanny Tzelepis, Julie Joseph, Elias K. Haddad, Susanne MacLean, Renu Dudani, Fabien Agenes, Stanford L. Peng, Rafick-Pierre Sekaly and Subash Sad
The Journal of immunology (1950), v 190(3), pp 1066-1075
01 Feb 2013
PMID: 23277488
url
https://www.jimmunol.org/content/jimmunol/190/3/1066.full.pdfView
Published, Version of Record (VoR) Open

Abstract

Immunology Life Sciences & Biomedicine Science & Technology
CD8(+) T cells undergo rapid expansion during infection with intracellular pathogens, which is followed by swift and massive culling of primed CD8(+) T cells. The mechanisms that govern the massive contraction and maintenance of primed CD8(+) T cells are not clear. We show in this study that the transcription factor, FoxO3a, does not influence Ag presentation and the consequent expansion of CD8(+) T cell response during Listeria monocytogenes infection, but plays a key role in the maintenance of memory CD8(+) T cells. The effector function of primed CD8(+) T cells as revealed by cytokine secretion and CD107a degranulation was not influenced by inactivation of FoxO3a. Interestingly, FoxO3a-deficient CD8(+) T cells displayed reduced expression of proapoptotic molecules BIM and PUMA during the various phases of response, and underwent reduced apoptosis in comparison with wild-type cells. A higher number of memory precursor effector cells and memory subsets was detectable in FoxO3a-deficient mice compared with wild-type mice. Furthermore, FoxO3a-deficient memory CD8(+) T cells upon transfer into normal or RAG1-deficient mice displayed enhanced survival. These results suggest that FoxO3a acts in a cell-intrinsic manner to regulate the survival of primed CD8(+) T cells. The Journal of Immunology, 2013, 190: 1066-1075.

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