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Accepted (AM)Open Access (License Unspecified), Open
Journal article
Intrinsic and growth-mediated cell and matrix specialization during murine meniscus tissue assembly
The FASEB journal, v 35(8), pp e21779-n/a
Aug 2021
PMID: 34314047
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The incredible mechanical strength and durability of mature fibrous tissues and their extremely limited turnover and regenerative capacity underscores the importance of proper matrix assembly during early postnatal growth. In tissues with composite extracellular matrix (ECM) structures, such as the adult knee meniscus, fibrous (Collagen-I rich), and cartilaginous (Collagen-II, proteoglycan-rich) matrix components are regionally segregated to the outer and inner portions of the tissue, respectively. While this spatial variation in composition is appreciated to be functionally important for resisting complex mechanical loads associated with gait, the establishment of these specialized zones is poorly understood. To address this issue, the following study tracked the growth of the murine meniscus from its embryonic formation through its first month of growth, encompassing the critical time-window during which animals begin to ambulate and weight bear. Using histological analysis, region specific high-throughput qPCR, and Col-1, and Col-2 fluorescent reporter mice, we found that matrix and cellular features defining specific tissue zones were already present at birth, before continuous weight-bearing had occurred. These differences in meniscus zones were further refined with postnatal growth and maturation, resulting in specialization of mature tissue regions. Taken together, this work establishes a detailed timeline of the concurrent spatiotemporal changes that occur at both the cellular and matrix level throughout meniscus maturation. The findings of this study provide a framework for investigating the reciprocal feedback between cells and their evolving microenvironments during assembly of a mechanically robust fibrocartilage tissue, thus providing insight into mechanisms of tissue degeneration and effective regenerative strategies.
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Details
- Title
- Intrinsic and growth-mediated cell and matrix specialization during murine meniscus tissue assembly
- Creators
- Tonia K Tsinman - University of PennsylvaniaXi Jiang - University of PennsylvaniaLin Han - School of Biomedical Engineering, Science and Health Systems, Drexel University, Philadelphia, PA, USAEiki Koyama - Children's Hospital of PhiladelphiaRobert L Mauck - University of PennsylvaniaNathaniel A Dyment - University of Pennsylvania
- Publication Details
- The FASEB journal, v 35(8), pp e21779-n/a
- Publisher
- Wiley
- Grant note
- IK6 RX003416 / RRD VA T32 AR007132 / NIAMS NIH HHS P30 AR069619 / NIAMS NIH HHS R01 AR075418 / NIAMS NIH HHS S10 OD021633 / NIH HHS R00 AR067283 / NIAMS NIH HHS
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000678975300035
- Scopus ID
- 2-s2.0-85111547584
- Other Identifier
- 991019169575904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology