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Journal article
Investigating the distribution of HIV-1 Tat lengths present in the Drexel Medicine CARES cohort
Virus research, v 272, 197727
15 Oct 2019
PMID: 31437485
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
•Accumulation of HIV-1 Tat truncations was observed in both CARES and LANL.•Most Tat sequences in the CARES Cohort and LANL database were 101 residues long.•No correlation was observed between Tat length and clinical parameters within CARES.•Longitudinal analysis showed a high likelihood of Tat 86 or 101 occurrence after visits where Tat was below 86 residues.•Specific Tat truncations appear to transactivate the HIV-1 LTR potentially due to stop codon read through.
Human immunodeficiency virus type 1 (HIV-1) encodes for Tat, a multi-functional regulatory protein involved in transcriptional enhancement and in causing neurotoxicity/central nervous system (CNS) dysfunction. This study examines Sanger sequencing of HIV-1 subtype B Tat from 2006 to 2014 within the Drexel University College of Medicine CNS AIDS Research and Eradication Study (CARES) Cohort to investigate Tat length in patients. The Los Alamos National Laboratory (LANL) database was used as a comparator. Miscoded stop codons were present in the CARES Cohort and LANL and protein variability was highly similar. Tat proteins in CARES and LANL were predominantly 101 residues. There was no observed correlation between Tat length and clinical parameters within the CARES Cohort. Unique Tat lengths found in the CARES Cohort and not in LANL were 31, 36, and 39 residues. When CARES patients were longitudinally examined, sequence lengths of 101 had a low probability of reducing to below 48, and sequences had a high probability of increasing to above 86 residues during their next visit, when below 48 residues in length. This suggests that Tat length is conserved to retain the majority of the proteins function highlighting its importance in viral replication.
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Details
- Title
- Investigating the distribution of HIV-1 Tat lengths present in the Drexel Medicine CARES cohort
- Creators
- Robert W. Link - Drexel UniversityAnthony R. Mele - Drexel UniversityGregory C. Antell - Drexel UniversityVanessa Pirrone - Drexel UniversityWen Zhong - Drexel UniversityKatherine Kercher - Drexel UniversityShendra Passic - Drexel UniversityZsofia Szep - Drexel UniversityKim Malone - Drexel UniversityJeffrey M. Jacobson - Temple UniversityWill Dampier - Drexel UniversityBrian Wigdahl - Drexel UniversityMichael R. Nonnemacher - Drexel University
- Publication Details
- Virus research, v 272, 197727
- Publisher
- Elsevier
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology; Infectious Diseases (and HIV Medicine)
- Web of Science ID
- WOS:000487572900014
- Scopus ID
- 2-s2.0-85071398221
- Other Identifier
- 991019167637404721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology