Journal article
Investigation of Plasma Biomarkers in HIV-1/HCV Mono- and Coinfected Individuals by Multiplex iTRAQ Quantitative Proteomics
Omics (Larchmont, N.Y.), v 15(10), pp 75-717
01 Oct 2011
PMID: 21978398
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The analysis of plasma samples from HIV-1/HCV mono- and coinfected individuals by quantitative proteomics is an efficient strategy to investigate changes in protein abundances and to characterize the proteins that are the effectors of cellular functions involved in viral pathogenesis. In this study, the infected and healthy plasma samples (in triplicate) were treated with ProteoMiner beads to equalize protein concentrations and subjected to 4-plex iTRAQ labeling and liquid chromatography/mass spectrometry (LC-MS/MS) analysis. A total of 70 proteins were identified with high confidence in the triplicate analysis of plasma proteins and 65% of the proteins were found to be common among the three replicates. Apolipoproteins and complement proteins are the two major classes of proteins that exhibited differential regulation. The results of quantitative analysis revealed that APOA2, APOC2, APOE, C3, HRG proteins were upregulated in the plasma of all the three HIV-1 mono-, HCV mono-, and coinfected patient samples compared to healthy control samples. Ingenuity pathway analysis (IPA) of the upregulated proteins revealed that they are implicated in the hepatic lipid metabolism, inflammation, and acute-phase response signaling pathways. Thus, we identified several differentially regulated proteins in HIV-1/HCV mono and coinfected plasma samples that may be potential biomarkers for liver disease.
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Details
- Title
- Investigation of Plasma Biomarkers in HIV-1/HCV Mono- and Coinfected Individuals by Multiplex iTRAQ Quantitative Proteomics
- Creators
- Vivekananda Shetty - 1Immunotope, Inc., Pennsylvania Biotechnology Center, Doylestown, PennsylvaniaPooja Jain - 2Drexel Institute for Biotechnology and Virology Research, and the Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaZacharie Nickens - 1Immunotope, Inc., Pennsylvania Biotechnology Center, Doylestown, PennsylvaniaGomathinayagam Sinnathamby - 1Immunotope, Inc., Pennsylvania Biotechnology Center, Doylestown, PennsylvaniaAnand Mehta - 2Drexel Institute for Biotechnology and Virology Research, and the Department of Microbiology and Immunology, Drexel University College of Medicine, Doylestown, PennsylvaniaRamila Philip - 1Immunotope, Inc., Pennsylvania Biotechnology Center, Doylestown, Pennsylvania
- Publication Details
- Omics (Larchmont, N.Y.), v 15(10), pp 75-717
- Publisher
- Mary Ann Liebert, Inc
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000295920500007
- Scopus ID
- 2-s2.0-80053995029
- Other Identifier
- 991014877830504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biotechnology & Applied Microbiology
- Genetics & Heredity