Journal article
Investigation of a new core-shell particle column for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides
Journal of pharmaceutical and biomedical analysis, v 96, pp 54-57
05 Aug 2014
PMID: 24727281
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A new core-shell particle column showed excellent performance and durability for separation of short (∼21-mer) ribonucleic acid (RNA) oligonucleotides by ion-pair reversed-phase liquid chromatography (IP-RPLC). Previously investigated core-shell C18 columns showed excellent peak shapes and separations of closely eluting impurities by IP-RPLC. However, these columns showed only modest long-term stability at the neutral pH and elevated column temperatures of ≥60°C, typically used for IP-RPLC analysis of oligonucleotides. The newly introduced SunShell C18 column provided separations comparable to the previously evaluated core-shell columns, but with significantly improved long-term column stability when operated at neutral pH and elevated column temperature.
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Details
- Title
- Investigation of a new core-shell particle column for ion-pair reversed-phase liquid chromatography analysis of oligonucleotides
- Creators
- Mirlinda Biba - Merck Research Laboratories, Rahway, NJ 07065, USA; Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA. Electronic address: mirlinda_biba@merck.comChristopher J Welch - Merck Research Laboratories, Rahway, NJ 07065, USAJoe P Foley - Department of Chemistry, Drexel University, Philadelphia, PA 19101, USA
- Publication Details
- Journal of pharmaceutical and biomedical analysis, v 96, pp 54-57
- Publisher
- Elsevier; England
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Chemistry
- Web of Science ID
- WOS:000336774400007
- Scopus ID
- 2-s2.0-84898634046
- Other Identifier
- 991014878119204721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Chemistry, Analytical
- Pharmacology & Pharmacy