Journal article
Investigation of bacterial chemotaxis using a simple three-point microfluidic system
Biochip journal, v 9(1), pp 50-58
01 Mar 2015
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
A three-point microfluidic system was developed and used to experimentally verify bacterial chemotaxis with known chemoeffectors. Using pneumatically-controlled micro-valves, the device was able to regulate microscale flows and created concentration gradients that allowed GFP-labelled Escherichia coli cells to interact with an environment that contained a chemoattractant and a chemorepellent. Having two separate possible paths (left and right) for the bacteria to move forward, this device also allowed for imaging processing based removal of noisy data, if adirectional bias was present. This device could be useful for quantitative analysis of chemotactic behaviors with minimal technical requirements, and could motivate the development of future devices based on this concept.
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Details
- Title
- Investigation of bacterial chemotaxis using a simple three-point microfluidic system
- Creators
- Hoyeon Kim - Drexel UniversityJamel Ali - Drexel UniversityKiran Phuyal - Drexel UniversitySungsu Park - Sungkyunkwan UniversityMin Jun Kim - Drexel University
- Publication Details
- Biochip journal, v 9(1), pp 50-58
- Publisher
- Korean Biochip Society-Kbcs
- Number of pages
- 9
- Grant note
- W911NF-11-1-0490 / Army Research Office 2012-00065 22 / NRF (National Research Foundation) Public Welfare AMP; Safety Research Program award in Korea
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000351872700007
- Scopus ID
- 2-s2.0-84925766498
- Other Identifier
- 991019173581104721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- Nanoscience & Nanotechnology