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Investigations of electrospray sample deposition for polymer MALDI mass spectrometry
Journal article   Open access

Investigations of electrospray sample deposition for polymer MALDI mass spectrometry

Scott D Hanton, Ingrid Z Hyder, James R Stets, Kevin G Owens, William R Blair, Charles M Guttman and Anthony A Giuseppetti
Journal of the American Society for Mass Spectrometry, v 15(2), pp 168-179
2004
PMID: 14766284
url
https://doi.org/10.1016/j.jasms.2003.09.012View
Published, Version of Record (VoR) Open

Abstract

In the interest of a more thorough understanding of the relationship between sample deposition technique and the quality of data obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, details of the electrospray (ES) process of sample deposition are investigated using a number of techniques. Sample morphology was observed with scanning electron microscopy (SEM) and atomic force microscopy (AFM), while matrix-enhanced secondary ion mass spectrometry (MESIMS) monitored surface coverage. Electrospray deposition reduces the analyte segregation that can occur during traditional dried droplet deposition for MALDI. We attribute statistically significant improvements in the reproducibility of signal intensity and MALDI average molecular mass measurements to the ES sample deposition technique.

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Collaboration types
Industry collaboration
Domestic collaboration
Web of Science research areas
Biochemical Research Methods
Chemistry, Analytical
Chemistry, Physical
Spectroscopy
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