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Isolated hepatocytes are capable of excreting aflatoxin metabolites
Journal article   Peer reviewed

Isolated hepatocytes are capable of excreting aflatoxin metabolites

John J. Ch'ih, Diane W. Biedrzycka and Thomas M. Devlin
Biochemical and biophysical research communications, v 178(3), pp 1002-1007
1991
DOI: https://doi.org/10.1016/0006-291X(91)90991-F
PMID: 1908229

Abstract

The intra- and extra- cellular concentrations of AFB 1, AFM 1, AFP 1, AFQ 1 and their conjugates were quantitatively determined after 60 min of incubation with [ 3H]-AFB 1 (1500 pmol/10 8 cells). Comparing the total concentrations of water-soluble conjugates, the eight fold greater amounts found in the medium (718 pmol) than in the cell (86 pmol) indicate that these detoxication products were excreted soon after they were formed. When the cells were perturbed with butylated hydroxyanisole (BHA), a noncompetitive inhibitor of the mixed-function oxidases, accumulations of intracellular AFB 1 and extracellular AFB 1 were observed. In a cell-free microsomal system, the AFB 1 was metabolized at a slower rate than in intact cells. When the activation of AFB 1 is blocked, the accumulation of intracellular AFB 1 and decreased internalization of AFB 1 suggest that AFB 1 uptake, translocation of AFB 1 from site of entry to site of actions, oxidation by cytochrome P-450-dependent monoxygenase, metabolic detoxication and conjugation reactions, and excretion of water soluble metabolites are linked.

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Web of Science research areas
Biochemistry & Molecular Biology
Biophysics
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