Journal article
Isolated single-lung perfusion with TNF-α in a rat sarcoma lung metastases model
The Annals of thoracic surgery, v 58(2), pp 328-332
01 Aug 1994
PMID: 8067827
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We conducted a trial of isolated lung perfusion using tumor necrosis factor (TNF) in an experimental sarcoma lung metastasis model. In an in vitro experiment, methylcholanthrene-induced sarcoma cells were incubated for 48 hours with 42 μg/mL of either human or murine TNF. Controls were incubated with Hank's balanced salt solution. In an in vivo experiment, 23 F344 rats were injected with 10
7 methylcholanthrene-induced sarcoma cells. On day 7, 4 animals were perfused with 210 μg of murine TNF, 5 animals were perfused with 420 μg of murine TNF, 10 animals underwent isolated lung perfusion with 420 μg of human TNF, and 4 animals were injected systemically with 420 μg of human TNF. Animals were sacrificed on day 14 and the lung nodules counted. The cells incubated with murine TNF exhibited a 21% decrease in growth (
p = 0.07); cells incubated with human TNF showed a 37% decrease in growth (
p < 0.05). Animals perfused with 210 μg/mL of murine TNF and animals treated by systemically administered human TNF showed no tumor response. Animals perfused with 420 μg/mL of murine TNF had 7.8 ± 14.2 nodules on the left lung and 58.5 ± 66.0 nodules on the right lung (
P = 0.07). Animals perfused with 420 μg/mL of human TNF had 21.7 ± 18.3 nodules on the left lung and 91.7 ± 66.2 nodules on the right lung (
p < 0.01). On the basis of these findings, we conclude that isolated lung perfusion with TNF can be done safely in the rat and is effective in decreasing the growth of sarcoma lung metastases.
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Details
- Title
- Isolated single-lung perfusion with TNF-α in a rat sarcoma lung metastases model
- Creators
- Benny Weksler - Memorial Sloan Kettering Cancer CenterDavid Blumberg - Memorial Sloan Kettering Cancer CenterJeffrey T. Lenert - Memorial Sloan Kettering Cancer CenterBruce Ng - Memorial Sloan Kettering Cancer CenterYuman Fong - Memorial Sloan Kettering Cancer CenterMichael E. Burt - Memorial Sloan Kettering Cancer Center
- Publication Details
- The Annals of thoracic surgery, v 58(2), pp 328-332
- Publisher
- Elsevier
- Number of pages
- 5
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Surgery
- Web of Science ID
- WOS:A1994PD58900010
- Scopus ID
- 2-s2.0-0028022108
- Other Identifier
- 991021960802804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cardiac & Cardiovascular Systems
- Respiratory System
- Surgery