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Isolating and targeting a highly active, stochastic dendritic cell subpopulation for improved immune responses
Journal article   Open access   Peer reviewed

Isolating and targeting a highly active, stochastic dendritic cell subpopulation for improved immune responses

Peter Deak, Bradley Studnitzer, Trevor Ung, Rachel Steinhardt, Melody Swartz and Aaron Esser-Kahn
Cell reports (Cambridge), v 41(5), pp 111563-111563
01 Nov 2022
PMID: 36323246
url
http://www.cell.com/article/S2211124722014243/pdfView
Published, Version of Record (VoR) Open
url
https://doi.org/10.1016/j.celrep.2022.111563View
Published, Version of Record (VoR) Open

Abstract

Cell Biology Life Sciences & Biomedicine Science & Technology
Dendritic cell (DC) activation via pathogen-associated molecular patterns (PAMPs) is critical for antigen pre-sentation and development of adaptive immune responses, but the stochastic distribution of DC responses to PAMP signaling, especially during the initial stages of immune activation, is poorly understood. In this study, we isolate a unique DC subpopulation via preferential phagocytosis of microparticles (MPs) and char-acterize this subpopulation of "first responders"(FRs). We present results that show these cells (1) can be isolated and studied via both increased accumulation of the micron-sized particles and combinations of cell surface markers, (2) show increased responses to PAMPs, (3) facilitate adaptive immune responses by providing the initial paracrine signaling, and (4) can be selectively targeted by vaccines to modulate both anti-body and T cell responses in vivo. This study presents insights into a temporally controlled, distinctive cell population that influences downstream immune responses. Furthermore, it demonstrates potential for improving vaccine designs via FR targeting.

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Collaboration types
Domestic collaboration
Web of Science research areas
Cell Biology
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