Journal article
Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse
International journal of systematic and evolutionary microbiology, v 66(9), pp 3585-3593
Sep 2016
PMID: 27298306
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Members of the genus Neisseria have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the Neisseria-specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus Neisseria from the oral cavity of the wild house mouse, Mus musculus subsp. domesticus. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named Neisseria musculi sp. nov. (type strain AP2031T=DSM 101846T=CCUG 68283T=LMG 29261T), was studied further. Strain AP2031T/N. musculi grew well in vitro. It was naturally competent, taking up DNA in a DNA uptake sequence and pilT-dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of N. musculi sp. nov. make it an ideal candidate for use in developing a mouse model for studying Neisseria-host interactions.
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Details
- Title
- Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse
- Creators
- Nathan J Weyand - University of ArizonaMancheong Ma - University of ArizonaMegan Phifer-Rixey - University of ArizonaNyiawung A Taku - University of ArizonaMaría A Rendón - University of ArizonaAlyson M Hockenberry - University of ArizonaWon J Kim - University of ArizonaAl B Agellon - University of ArizonaNicolas Biais - Brooklyn CollegeTaichi A Suzuki - University of ArizonaLily Goodyer-Sait - University of OxfordOdile B Harrison - University of OxfordHolly B Bratcher - University of OxfordMichael W Nachman - University of ArizonaMartin C J Maiden - University of OxfordMagdalene So - University of Arizona
- Publication Details
- International journal of systematic and evolutionary microbiology, v 66(9), pp 3585-3593
- Grant note
- R01 GM074245 / NIGMS NIH HHS Wellcome Trust 087622 / Wellcome Trust
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Biology
- Web of Science ID
- WOS:000386871200054
- Scopus ID
- 2-s2.0-84991065407
- Other Identifier
- 991021229983004721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Microbiology