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Isolation and characterization of PC-3 human prostatic tumor sublines which preferentially metastasize to select organs in S.C.I.D. mice
Journal article   Peer reviewed

Isolation and characterization of PC-3 human prostatic tumor sublines which preferentially metastasize to select organs in S.C.I.D. mice

Min Wang and Mark E. Stearns
Differentiation (London), v 48(2), pp 115-125
1991
DOI: https://doi.org/10.1111/j.1432-0436.1991.tb00250.x
PMID: 1773917
Featured in Collection :   UN Sustainable Development Goals @ Drexel

Abstract

We have developed and partially characterized a mouse model system for studying human prostate tumor cell metastases in vivo. To develop this model we have selected highly invasive (3 × 1.) and non-invasive (3 × N.I.) PC-3 human prostatic tumor sublines based on enhanced and diminished capacities to migrate across a reconstituted basement membrane barrier (Matrigel) in Boyden chamber chemotactic assays. When the 3 × 1 cells were injected intravenously (i.v.) in the tail vein of severe combined immune deficient (scid) mice, the cells initially metastasized to a wide variety of tissues as demonstrated by using [ 125I] IUdR labeled cells and histology. Four distinct sublines were eventually isolated which preferentially metastasized at ∼ 80% efficiency to the lumbar vertebrae (PC-3 ML), the mandibular region of the right cheek (PC-3 MC), the rib cartilage (PC-3 MR), and the right front knee bone (PC-3 MK), respectively. Implantation experiments at different sites indicated that organ metastases may somehow be conferred on the tumor subclones by the host tissue.

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121 citations in Web of Science
117 citations in Scopus

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Web of Science research areas
Cell Biology
Developmental Biology
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