Journal article
Isolation and chemical characterization of agelaiatoxin8 (AvTx8) from Agelaia vicina wasp venom and its biological effects on GABA neurotransmission
Journal of biochemical and molecular toxicology, v 31(10), pp e21941-n/a
Oct 2017
PMID: 28621878
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Arthropod venoms are sources of molecules that may be useful tools to investigate molecular mechanisms of putative new medicines and laboratory drugs. Here we show the effects of the compound agelaiatoxin‐8 (AVTx8), isolated from Agelaia vicina venom, on γ‐aminobutyric acid (GABA) neurotransmission in rat brain synaptosomes. Analysis reveals that AvTx8 is composed by 14 amino acid residues with a molecular weight (MW) of 1567 Da. AvTx8 increased GABA release and inhibited GABA uptake in synaptosomes from rat cerebral cortex. AvTx8 inhibited GABA uptake and increased GABA release in the presence of Ca+, Na+, and K+ channel blockers, suggesting that it acts directly on GABA transporters. In addition, AvTx8 significantly decreases GABA binding in synaptic membranes from rat brain cortex, suggesting that it also modulates the activity of GABA receptors. Moreover, AvTx8 decreased GAT‐1– and GAT‐3–mediated GABA uptake in transfected COS‐7 cells. Accordingly, we suggest that AvTx8 modulates GABA neurotransmission and might provide a novel entry point for identifying a new class of GABA‐modulating neuroprotective drugs.
Metrics
Details
- Title
- Isolation and chemical characterization of agelaiatoxin8 (AvTx8) from Agelaia vicina wasp venom and its biological effects on GABA neurotransmission
- Creators
- Andrea B. Pizzo - Universidade de São PauloRenê O. Beleboni - Universidade de Ribeirão PretoRuither O. Gomes Carolino - University of Sao PauloLuciana Oliveira - University of Sao PauloAntonio Miranda - Universidade Federal de São PauloJoaquim Coutinho-Netto - Universidade de São PauloAndréia C. K. Fontana - Drexel UniversityWagner Ferreira dos Santos - Universidade de São Paulo
- Publication Details
- Journal of biochemical and molecular toxicology, v 31(10), pp e21941-n/a
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- Fundação de Amparo à Pesquisa do Estado de São Paulo (00/08010‐5; 00/08101‐0)
- Resource Type
- Journal article
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000412328200001
- Scopus ID
- 2-s2.0-85021414888
- Other Identifier
- 991020099208704721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Toxicology